学歴
2003 東京大学薬学部薬学科卒業
2008 同大学院修了、博士(薬学)
職歴
2005 - 2008 日本学術振興会特別研究員(DC1)
2008 - 2011 大阪大学蛋白質研究所・助教
2011 - 2022 大阪大学微生物病研究所・助教
2022 - 現在 大阪大学微生物病研究所・准教授
特記事項
2010 第62回日本細胞生物学会・若手優秀発表賞受賞
2010 大阪大学「飛翔30」若手プログラム・研究フェロー選出
研究業績
- Lohani, S., Funato, Y., Akieda, Y., Mizutani, K., Takai, Y., Ishitani, T., and Miki, H.
A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence.
J. Cell Sci. 135, jcs258550 (2022)
- #Funato, Y. and #Miki, H.(#: co-correspondence)
The emerging roles and therapeutic potential of cyclin M/CorC family of Mg2+ transporters.
J. Pharmacol. Sci. 148, 14-18 (2022)
- Funato, Y., Yamazaki, D., Okuzaki, D., Yamamoto, N., and Miki, H.
Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.
Nat. Commun. 12, 3683 (2021)
- Huang, Y., Mu, K., Teng, X., Zhao, Y., Funato, Y., Miki, H., Zhu, W., Xu, Z., and Hattori, M.
Identification and mechanistic analysis of an inhibitor of the CorC Mg2+ transporter.
iScience 24, 102370 (2021)
- Yamazaki, D., Hashizume, O., Taniguchi, S., Funato, Y., and Miki, H.
Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture.
Sci. Rep. 11, 3980 (2021)
- #Huang, Y., #Jin, F., #Funato, Y., Xu, Z., Zhu, W., Wang, J., Sun, M., Zhao, Y., Yu, Y., Miki, H., and Hattori, M. (#: equal contribution)
Structural basis for the Mg2+ recognition and regulation of the CorC Mg2+ transporter.
Science Advances 7, eabe6140 (2021)
- Wu, Y., Funato, Y., Meschi, E., Jovanoski, K., Miki, H., and Waddell, S.
Magnesium efflux from Drosophila Kenyon Cells is critical for normal and diet-enhanced long-term memory.
eLife 9, e61339 (2020)
- #Funato, Y., Yoshida, A., Hirata, Y., Hashizume, O., Yamazaki, D., and #Miki, H.(#: co-correspondence)
The oncogenic PRL protein causes acid addiction of cells by stimulating lysosomal exocytosis.
Dev. Cell 55, 387-397 (2020)
- #Kozlov, G., #Funato, Y., Chen, Y.S., Zhang, Z., Illes, K., Miki, H., and Gehring, K. (#: equal contribution)
PRL3 pseudophosphatase activity is necessary and sufficient to promote metastatic growth.
J. Biol. Chem. 295, 11682-11692 (2020)
- Hashizume, O., #Funato, Y., Yamazaki, D., and #Miki, H.(#: co-correspondence)
Excessive Mg2+ Impairs Intestinal Homeostasis by Enhanced Production of Adenosine Triphosphate and Reactive Oxygen Species.
Antioxid. Redox Signal. 33, 20-34 (2020)
- Dóka, É., Ida, T., Dagnell, M., Abiko, Y., Luong, N.C., Balog, N., Takata, T., Espinosa, B., Nishimura, A., Cheng, Q., Funato, Y., Miki, H., Fukuto, J.M., Prigge, J.R., Schmidt, E.E., Arnér, E.S.J., Kumagai, Y., Akaike, T., and Nagy, P.
Control of protein function through oxidation and reduction of persulfidated states.
Science Advances 6, eaax8358 (2020)
- Kojima, T., #Funato, Y., and #Miki, H.(#: co-correspondence)
Phosphatase of regenerating liver sensitizes MET to functional activation by hepatocyte growth factor.
Biochem. J. 476, 1419-1431 (2019)
- Yamazaki, D., Hasegawa, A., Funato, Y., Tran, H.N., Mori, M.X., Mori, Y., Sato, T., and Miki, H.
Cnnm4 deficiency suppresses Ca2+ signaling and promotes cell proliferation in the colon epithelia.
Oncogene 38, 3962-3969 (2019)
- Funato, Y. and Miki, H.
Molecular function and biological importance of CNNM family Mg2+ transporters.
J. Biochem. 165, 219-225 (2019)
- Chen, Y.S., Kozlov, G., Fakih, R., Funato, Y., Miki, H., and Gehring, K.
The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity.
J. Biol. Chem. 293, 19998-20007 (2018)
- Yoshida, A., Funato, Y., and Miki, H.
Phosphatase of regenerating liver maintains cellular magnesium homeostasis.
Biochem. J. 475, 1129-1139 (2018)
- #Funato, Y., Furutani, K., Kurachi, Y., and #Miki, H.(#: co-correspondence)
CNNM proteins are Na+/Mg2+ exchangers playing a central role in transepithelial Mg2+ (re)absorption.
J. Physiol. 596, 743-746 (2018)
- Matsui, Y., Funato, Y., Imamura, H., Miki, H., Mizukami, S., and Kikuchi, K.
Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe.
Chem. Sci. 8, 8255-8264 (2017)
- Funato, Y., Yamazaki, D., and Miki, H.
Renal function of cyclin M2 Mg2+ transporter maintains blood pressure.
J. Hypertens. 35, 585-592 (2017)
- #Gulerez, I., #Funato, Y., #Wu, H., Yang, M., Kozlov, G., Miki, H., and Gehring, K. (#: equal contribution)
Phosphocysteine in the PRL-CNNM pathway mediates magnesium homeostasis.
EMBO Rep. 17, 1890-1900 (2016)
- Ishii, T., #Funato, Y., Hashizume, O., Yamazaki, D., Hirata, Y., Nishiwaki, K., Kono, N., Arai, H., and #Miki, H. (#: co-correspondence)
Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
PLoS Genet. 12, e1006276 (2016)
- Yamazaki, D., Funato, Y., Miyata, H., Ikawa, M., and Miki, H.
Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation.
Biochem. Biophys. Res. Commun. 474, 441-446 (2016)
- Yamazaki, D., Miyata, H., Funato, Y., Fujihara, Y., Ikawa, M., and Miki, H.
Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.
J. Cell Sci. 129, 1940-1949 (2016)
- Hirata, Y., Funato, Y., and Miki, H.
Basolateral sorting of the Mg(2+) transporter CNNM4 requires interaction with AP-1A and AP-1B.
Biochem. Biophys. Res. Commun. 455, 184-189 (2014)
- Funato, Y., Yamazaki, D., Mizukami, S., Du, L., Kikuchi, K., and Miki, H.
Membrane protein CNNM4-dependent Mg2+ efflux suppresses tumor progression.
J. Clin. Invest. 124, 5398-5410 (2014)
- Yugi, K., Kubota, H., Toyoshima, Y., Noguchi, R., Kawata, K., Komori, Y., Uda, S., Kunida, K., Tomizawa, Y., Funato, Y., Miki, H., Matsumoto, M., Nakayama, K.I., Kashikura, K., Endo, K., Ikeda, K., Soga, T., and Kuroda, S.
Reconstruction of insulin signal flow from phosphoproteome and metabolome data.
Cell Rep. 8, 1171–1183 (2014)
- Hirata, Y., Funato, Y., Takano, Y., and Miki, H.
Mg2+-dependent interactions of ATP with the cystathionine-β-synthase (CBS) domains of a magnesium transporter.
J. Biol. Chem. 289, 14731-14739 (2014)
- Funato, Y. and Miki, H.
Reversible oxidation of PRL family protein-tyrosine phosphatases.
Methods 65, 184-189 (2014)
- #Yamazaki, D., #Funato, Y., Miura, J., Sato, S., Toyosawa, S., Furutani, K., Kurachi, Y., Omori, Y., Furukawa, T., Tsuda, T., Kuwabata, S., Mizukami, S., Kikuchi, K., and Miki, H. (#: equal contribution)
Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model.
PLoS Genet. 9, e1003983 (2013)
- Funato, Y., Hayashi, T., Irino, Y., Takenawa, T., and Miki, H.
Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1.
Biochem. Biophys. Res. Commun. 440, 737-742 (2013)
- Ishii, T., Funato, Y., and Miki, H.
Thioredoxin-related protein 32 (TRP32) specifically reduces oxidized phosphatase of regenerating liver (PRL).
J. Biol. Chem. 288, 7263–7270 (2013)
- Miki, H., and Funato, Y.
Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species.
J. Biochem. 151, 255–261 (2012)
- Morinaka, A., Funato, Y., Uesugi, K., and Miki, H.
Oligomeric peroxiredoxin-I is an essential intermediate for p53 to activate MST1 kinase and apoptosis.
Oncogene 30, 4208-4218 (2011)
- Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H.
Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse.
Science Signal. 4, ra26 (2011)
- Müller, L., Funato, Y., Miki, H., and Zimmermann, R.
An interaction between human Sec63 and nucleoredoxin may provide the missing link between the SEC63 gene and polycystic liver disease.
FEBS Lett. 585, 596-600 (2011)
- Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H., Yoshida, N., and Miki, H.
Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein.
Curr. Biol. 20, 1945-1952 (2010)
- #Hayashi, T., #Funato, Y., Tearabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., and Miki, H. (#: equal contribution)
Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I toMyeloid differentiation primary response gene (88).
J. Biol. Chem. 285, 18586-18593 (2010)
- #Funato, Y., and #Miki, H. (#: co-correspondence)
Redox regulation of Wnt signalling via nucleoredoxin.
Free Radic. Res. 44, 379-388 (2010)
- Boles, M. K., Wilkinson, B. M., Wilming, L., Liu, B., Probst, F. J., Harrow, J., Grafham, D., Hentges, K. E., Woodward, L. P., Maxwell, A., Mitchell, K., Risley, M. D., Johnson, R., Hirschi, K., Lupski, J. R., Funato, Y., Miki, H., Marcin-Garcia, P., Mattews, L., Coffey, A. J., Parker, A., Hubbard, T. J., Rogers, J., Bradley, A., Adams, D. J., and Justice, M. J.
Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing identifies a splice-site mutation in nucleoredoxin.
PLoS Genet. 5, e1000759 (2009)
- Terabayashi, T., Funato, Y., Fukuda, M., and Miki, H.
A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
J. Biol. Chem. 284, 26716-26724 (2009)
- Terabayashi, T., Funato, Y., and Miki, H.
Dishevelled-induced phosphorylation regulates membrane localization of Par1b.
Biochem. Biophys. Res. Commun. 375, 660-665 (2008)
- Funato, Y., Michiue, T., Terabayashi, T., Yukita, A., Danno, H., Asashima, M., andMiki, H.
Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus.
Genes. Cells. 13, 965-975 (2008)
- Terabayashi, T., Itoh, T. J., Yamaguchi, H., Yoshimura, Y., Funato, Y., Ohno, S., and Miki, H.
Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons.
J. Neurosci. 27,13098-13107 (2007)
- Funato, Y., and Miki, H.
Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation.
Antioxid. Redox Signal. 9, 1035-1057 (2007)
- Funato, Y., Michiue, T., Asashima, M., and Miki, H.
The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-β-catenin signaling through Dishevelled.
Nat. Cell Biol. 8, 501-508 (2006)
- Funato, Y., Terabayashi, T., Suenaga, N., Seiki, M., Takenawa, T., and Miki, H.
IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.
Cancer Res. 64, 5237-5244 (2004)