学歴
1993 東京大学理学部生物化学科卒業
1998 同大学院修了、博士(理学)
職歴
1995 - 1998 日本学術振興会特別研究員(DC1)
1998 - 2002 東京大学医科学研究所・助手
2002 - 2007 東京大学医科学研究所・助教授
2007 - 2011 大阪大学蛋白質研究所・教授
2011 - 現在 大阪大学微生物病研究所・教授
特記事項
2001 日本生化学会奨励賞受賞
2000 - 2003 科学技術振興機構戦略的創造研究推進事業さきがけ「タイムシグナルと制御」領域研究員(兼任)
2003 - 2007 科学技術振興機構戦略的創造研究推進事業さきがけ「情報と細胞機能」領域研究員(兼任)
研究業績
- Lohani, S., Funato, Y., Akieda, Y., Mizutani, K., Takai, Y., Ishitani, T., and Miki, H.
A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence.
J. Cell Sci. 135, jcs258550 (2022)
- Funato, Y. and Miki, H.
The emerging roles and therapeutic potential of cyclin M/CorC family of Mg2+ transporters.
J. Pharmacol. Sci. 148, 14-18 (2022)
- Okumura, S., Konishi, Y., Narukawa, M., Sugiura, Y., Yoshimoto, S., Arai, Y., Sato, S., Yoshida, Y., Tsuji, S., Uemura, K., Wakita, M., Matsudaira, T., Matsumoto, T., Kawamoto, S., Takahashi, A., Itatani, Y., Miki, H., Takamatsu, M., Obama, K., Takeuchi, K., Suematsu, M., Ohtani, N., Fukunaga, Y., Ueno, M., Sakai, Y., Nagayama, S., and Hara, E.
Gut bacteria identified in colorectal cancer patients promote tumourigenesis via butyrate secretion.
Nat. Commun. 12, 5674 (2021)
- Funato, Y., Yamazaki, D., Okuzaki, D., Yamamoto, N., and Miki, H.
Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.
Nat. Commun. 12, 3683 (2021)
- Huang, Y., Mu, K., Teng, X., Zhao, Y., Funato, Y., Miki, H., Zhu, W., Xu, Z., and Hattori, M.
Identification and mechanistic analysis of an inhibitor of the CorC Mg2+ transporter.
iScience 24, 102370 (2021)
- Yamazaki, D., Hashizume, O., Taniguchi, S., Funato, Y., and Miki, H.
Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture.
Sci. Rep. 11, 3980 (2021)
- Gehring, K. and Miki, H.
Phosphatase, pseudo-phosphatase, or both? Understanding PRL oncogenicity.
Br. J. Cancer 124, 1035–1036 (2021)
- Huang, Y., Jin, F., Funato, Y., Xu, Z., Zhu, W., Wang, J., Sun, M., Zhao, Y., Yu, Y., #Miki, H., and #Hattori, M. (#: co-correpondence)
Structural basis for the Mg2+ recognition and regulation of the CorC Mg2+ transporter.
Science Advances 7, eabe6140 (2021)
- Wu, Y., Funato, Y., Meschi, E., Jovanoski, K., Miki, H., and Waddell, S.
Magnesium efflux from Drosophila Kenyon Cells is critical for normal and diet-enhanced long-term memory.
eLife 9, e61339 (2020)
- Funato, Y., Yoshida, A., Hirata, Y., Hashizume, O., Yamazaki, D., and Miki, H.
The oncogenic PRL protein causes acid addiction of cells by stimulating lysosomal exocytosis.
Dev. Cell 55, 387-397 (2020)
- Kozlov, G., Funato, Y., Chen, Y.S., Zhang, Z., Illes, K., Miki, H., and Gehring, K.
PRL3 pseudophosphatase activity is necessary and sufficient to promote metastatic growth.
J. Biol. Chem. 295, 11682-11692 (2020)
- Ito, S., Nada, S., Yamazaki, D., Kimura, T., Kajiwara, K., Miki, H., and Okada, M.
p18/Lamtor1-mTORC1 signaling controls development of mucin-producing goblet cells in the intestinal epithelium.
Cell Struct. Funct. 45, 93-105 (2020)
- Matsushita, M., Nakamura, T., Moriizumi, H., Miki, H., and Takekawa, M.
Stress-responsive MTK1 SAPKKK serves as a redox sensor that mediates delayed and sustained activation of SAPKs by oxidative stress.
Science Advances 6, eaay9778 (2020)
- Hashizume, O., Funato, Y., Yamazaki, D., and Miki, H.
Excessive Mg2+ Impairs Intestinal Homeostasis by Enhanced Production of Adenosine Triphosphate and Reactive Oxygen Species.
Antioxid. Redox Signal. 33, 20-34 (2020)
- Dóka, É., Ida, T., Dagnell, M., Abiko, Y., Luong, N.C., Balog, N., Takata, T., Espinosa, B., Nishimura, A., Cheng, Q., Funato, Y., Miki, H., Fukuto, J.M., Prigge, J.R., Schmidt, E.E., Arnér, E.S.J., Kumagai, Y., Akaike, T., and Nagy, P.
Control of protein function through oxidation and reduction of persulfidated states.
Science Advances 6, eaax8358 (2020)
- Kojima, T., Funato, Y., and Miki, H.
Phosphatase of regenerating liver sensitizes MET to functional activation by hepatocyte growth factor.
Biochem. J. 476, 1419-1431 (2019)
- Yamazaki, D., Hasegawa, A., Funato, Y., Tran, H.N., Mori, M.X., Mori, Y., Sato, T., and Miki, H.
Cnnm4 deficiency suppresses Ca2+ signaling and promotes cell proliferation in the colon epithelia.
Oncogene 38, 3962-3969 (2019)
- Funato, Y. and Miki, H.
Molecular function and biological importance of CNNM family Mg2+ transporters.
J. Biochem. 165, 219-225 (2019)
- Chen, Y.S., Kozlov, G., Fakih, R., Funato, Y., Miki, H., and Gehring, K.
The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity.
J. Biol. Chem. 293, 19998-20007 (2018)
- Yoshida, A., Funato, Y., and Miki, H.
Phosphatase of regenerating liver maintains cellular magnesium homeostasis.
Biochem. J. 475, 1129-1139 (2018)
- Funato, Y., Furutani, K., Kurachi, Y., and Miki, H.
CNNM proteins are Na+/Mg2+ exchangers playing a central role in transepithelial Mg2+ (re)absorption.
J. Physiol. 596, 743-746 (2018)
- Matsui, Y., Funato, Y., Imamura, H., Miki, H., Mizukami, S., and Kikuchi, K.
Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe.
Chem. Sci. 8, 8255-8264 (2017)
- Funato, Y., Yamazaki, D., and Miki, H.
Renal function of cyclin M2 Mg2+ transporter maintains blood pressure.
J. Hypertens. 35, 585-592 (2017)
- Gulerez, I., Funato, Y., Wu, H., Yang, M., Kozlov, G., #Miki, H., and #Gehring, K. (#: co-correpondence)
Phosphocysteine in the PRL-CNNM pathway mediates magnesium homeostasis.
EMBO Rep. 17, 1890-1900 (2016)
- Ishii, T., Funato, Y., Hashizume, O., Yamazaki, D., Hirata, Y., Nishiwaki, K., Kono, N., Arai, H., and Miki, H.
Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
PLoS Genet. 12, e1006276 (2016)
- Yamazaki, D., Funato, Y., Miyata, H., Ikawa, M., and Miki, H.
Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation.
Biochem. Biophys. Res. Commun. 474, 441-446 (2016)
- Yamazaki, D., Miyata, H., Funato, Y., Fujihara, Y., Ikawa, M., and Miki, H.
Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.
J. Cell Sci. 129, 1940-1949 (2016)
- Hirata, Y., Funato, Y., and Miki, H.
Basolateral sorting of the Mg(2+) transporter CNNM4 requires interaction with AP-1A and AP-1B.
Biochem. Biophys. Res. Commun. 455, 184-189 (2014)
- Funato. Y., Yamazaki, D., Mizukami, S., Du, L., Kikuchi, K., and Miki, H.
Membrane protein CNNM4-dependent Mg2+ efflux suppresses tumor progression.
J. Clin. Invest. 124, 5398-5410 (2014)
- Yugi, K., Kubota, H., Toyoshima, Y., Noguchi, R., Kawata, K., Komori, Y., Uda, S., Kunida, K., Tomizawa, Y., Funato, Y., Miki, H., Matsumoto, M., Nakayama, K.I., Kashikura, K., Endo, K., Ikeda, K., Soga, T., and Kuroda, S.
Reconstruction of insulin signal flow from phosphoproteome and metabolome data.
Cell Rep. 8, 1171–1183 (2014)
- Hirata, Y., Funato, Y., Takano, Y., and Miki, H.
Mg2+-dependent interactions of ATP with the cystathionine-β-synthase (CBS) domains of a magnesium transporter.
J. Biol. Chem. 289, 14731-14739 (2014)
- Funato, Y. and Miki, H.
Reversible oxidation of PRL family protein-tyrosine phosphatases.
Methods 65, 184-189 (2014)
- Yamazaki, D., Funato, Y., Miura, J., Sato, S., Toyosawa, S., Furutani, K., Kurachi, Y., Omori, Y., Furukawa, T., Tsuda, T., Kuwabata, S., Mizukami, S., Kikuchi, K., and Miki, H.
Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model.
PLoS Genet. 9, e1003983 (2013)
- Funato, Y., Hayashi, T., Irino, Y., Takenawa, T., and Miki, H.
Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1.
Biochem. Biophys. Res. Commun. 440, 737-742 (2013)
- Yamazaki, D., Itoh, T., Miki, H., and Takenawa, T.
srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity.
Mol. Biol. Cell 24, 3393–3405 (2013)
- Ishii, T., Funato, Y., and Miki, H.
Thioredoxin-related protein 32 (TRP32) specifically reduces oxidized phosphatase of regenerating liver (PRL).
J. Biol. Chem. 288, 7263–7270 (2013)
- Terabayashi, T., Sakaguchi, M., Shinmyozu, K., Ohshima, T., Johjima, A., Ogura, T., Miki, H., and Nishinakamura, R.
Phosphorylation of Kif26b promotes its polyubiquitination and subsequent proteasomal degradation during kidney development.
PLoS One 7, e39714 (2012)
- Miki, H., and Funato, Y.
Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species.
J. Biochem. 151, 255–261 (2012)
- Morinaka, A., Funato, Y., Uesugi, K., and Miki, H.
Oligomeric peroxiredoxin I is an essential intermediate for p53 to activate MST1 kinase and apoptosis.
Oncogene 30, 4208-4218 (2011)
- Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H.
Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse.
Science Signal. 4, ra26 (2011)
- Yoshimura, Y., and Miki, H.
Dynamic regulation of GEF-H1 localization at microtubules by Par1b/MARK2.
Biochem. Biophys. Res. Commun. 408, 322-328 (2011)
- Müller, L., Funato, Y., Miki, H., and Zimmermann, R.
An interaction between human Sec63 and nucleoredoxin may provide the missing link between the SEC63 gene and polycystic liver disease.
FEBS Lett. 585, 596-600 (2011)
- Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H., Yoshida, N., and Miki, H.
Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein.
Curr. Biol. 20, 1945-1952 (2010)
- Hayashi, T., Funato, Y., Tearabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., and Miki, H.
Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I to Myeloid differentiation primary response gene (88).
J. Biol. Chem. 285, 18586-18593 (2010)
- Uchiyama, Y., Sakaguchi, M., Terabayashi, T., Inenaga, T., Inoue, S., Kobayashi, C., Oshima, N., Kiyonari, H., Nakagata, N., Sato, Y., Sekiguchi, K., Miki, H., Araki, E., Fujimura, S., Tanaka, S., and Nishinakamura, R.
Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme.
Proc. Natl. Acad. Sci. U.S.A. 107, 9240-9245 (2010)
- Yoshimura, Y., Terabayashi, T., and Miki, H.
Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation.
Mol. Cell. Biol. 30, 2206-2219 (2010)
- Funato, Y., and Miki, H.
Redox regulation of Wnt signalling via nucleoredoxin.
Free Radic. Res. 44, 379-388 (2010)
- Boles, M. K., Wilkinson, B. M., Wilming, L., Liu, B., Probst, F. J., Harrow, J., Grafham, D., Hentges, K. E., Woodward, L. P., Maxwell, A., Mitchell, K., Risley, M. D., Johnson, R., Hirschi, K., Lupski, J. R., Funato, Y., Miki, H., Marcin-Garcia, P., Mattews, L., Coffey, A. J., Parker, A., Hubbard, T. J., Rogers, J., Bradley, A., Adams, D. J., and Justice, M. J.
Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing identifies a splice-site mutation in nucleoredoxin.
PLoS Genet. 5, e1000759 (2009)
- Terabayashi, T., Funato, Y., Fukuda, M., and Miki, H.
A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
J. Biol. Chem. 284, 26716-26724 (2009)
- Kise, Y., Morinaka, A., Teglund, S., and Miki, H.
Sufu recruits GSK3beta for efficient processing of Gli3.
Biochem. Biophys. Res. Commun. 387, 569-574 (2009)
- Terabayashi, T., Funato, Y., and Miki, H.
Dishevelled-induced phosphorylation regulates membrane localization of Par1b.
Biochem. Biophys. Res. Commun. 375, 660-665 (2008)
- Funato, Y., Michiue, T., Terabayashi, T., Yukita, A., Danno, H., Asashima, M., and Miki, H.
Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus.
Genes. Cells. 13, 965-75 (2008)
- Terabayashi, T., Itoh, T. J., Yamaguchi, H., Yoshimura, Y., Funato, Y., Ohno, S., and Miki, H.
Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons.
J. Neurosci. 27,13098-13107 (2007)
- Funato, Y., and Miki, H.
Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation.
Antioxid. Redox Signal. 9, 1035-57. (2007)
- Takenaka, K., Kise, Y., and Miki, H.
GSK3beta positively regulates Hedgehog signaling through Sufu in mammalian cells.
Biochem. Biophys. Res. Commun. 353, 501-508 (2007)
- Tsuchiya, D., Kitamura, Y., Takata, K., Taniguchi, T., Uemura, K., Miki, H., Takenawa, T., and Shimohama, S.
Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells.
J. Pharmacol. Sci. 102, 354-358 (2006)
- Kise, Y., Takenaka, K., Tezuka, T., Yamamoto, T., and Miki, H.
Fused kinase is stabilized by Cdc37/Hsp90 and enhances Gli protein levels.
Biochem. Biophys. Res. Commun. 351, 78-84 (2006)
- Tsuchiya, D., Kitamura, Y., Takata, K., Sugisaki, T., Taniguchi, T., Uemura, K., Miki, H., Takenawa, T., and Shimohama, S.
Developmental expression of neural Wiskott-Aldrich syndrome protein (N-WASP) and WASP family verprolin-homologous protein
(WAVE)-related proteins in postnatal rat cerebral cortex and hippocampus.
Neurosci. Res. 56, 459-469 (2006)
- Funato, Y., Michiue, T., Asashima, M., and Miki, H.
The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-β-catenin signaling through Dishevelled.
Nat. Cell Biol. 8, 501-508 (2006)
- Oda, A., Miki, H., Wada, I., Yamaguchi, H., Yamazaki, D., Suetsugu, S., Nakajima, M., Nakayama, A., Okawa, K., Miyazaki, H., Matsuno, K., Ochs, H. D., Machesky, L. M., Fujita, H., and Takenawa, T.
WAVE/Scars in Platelets.
Blood 105, 3141-3148 (2005)
- Bierne, H., Miki, H., Innocenti, M., Scita, G., Gertler, F. B., Takenawa, T., and Cossart, P.
WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor.
J. Cell Sci. 118, 1537-1547 (2005)
- Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T., and Condeelis, J.
Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin.
J. Cell Biol. 168, 441-452 (2005)
- Kawamura, K., Takano, K., Suetsugu, S., Kurisu, S., Yamazaki, D., Miki, H., Takenawa, T., and Endo, T.
N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor
J. Biol. Chem. 279, 54862-54871 (2004)
- Verma, S., Shewan, A. M., Scott, J. A., Helwani, F. M., Den Elzen, N. R., Miki, H., Takenawa, T., and Yap, A. S.
Arp2/3 activity is necessary for efficient formation of E-cadherin adhesive contacts.
J. Biol. Chem. 279, 34062-34070 (2004)
- Funato, Y., Terabayashi, T., Suenaga, N., Seiki, M., Takenawa, T., and Miki, H.
IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.
Cancer Res. 64, 5237-5244 (2004)
- Takenaka, K., Nakagawa, H., Miyamoto, S., and Miki, H.
The pre-mRNA splicing factor SF3a66 functions as a microtubule binding and bundling protein.
Biochem. J. 382, 223-230 (2004)
- Miki, H., and Takenawa, T.
Regulation of actin dynamics by WASP family proteins.
J. Biochem. 134, 309-313 (2003)
- Yamazaki, D., Suetsugu, S., Miki, H., Kataoka, Y., Nishikawa, S., Fujiwara, T., Yoshida, N., and Takenawa, T.
WAVE2 is required for directed cell migration and cardiovascular development.
Nature 424, 452-456 (2003)
- Kitamura, Y., Tsuchiya, D., Takata, K., Shibagaki, K., Taniguchi, T., Smith, M. A., Perry, G., Miki, H., Takenawa, T., and Shimohama, S.
Possible involvement of Wiskott-Aldrich syndrome protein family in aberrant neuronal sprouting in Alzheimer's disease.
Neurosci. Lett. 346, 149-152 (2003)
- Kitamura, Y., Shibagaki, K., Takata, K., Tsuchiya, D., Taniguchi, T,. Gebicke-Haerter, P. J., Miki, H., Takenawa, T., and Shimohama, S
Involvement of Wiskott-Aldrich syndrome protein family verprolin-homologous protein (WAVE) and Rac1 in the phagocytosis of amyloid-beta(1 - 42) in rat microglia.
J. Pharmacol. Sci. 92, 115-123 (2003)
- Nakagawa, H., Miki, H., Nozumi, M., Takenawa, T., Miyamoto, S., Wehland, J., and Small, J. V.
IRSp53 is colocalised with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena.
J.Cell Sci. 116, 2577-2583 (2003)
- Sawa, M., Suetsugu, S., Sugimoto, A., Miki, H., Yamamoto, M., and Takenawa, T.
Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosure.
J. Cell Sci. 116, 1505-1518 (2003)
- Sun, P., Yamamoto, H., Suetsugu, S., Miki, H., Takenawa, T., and Endo, T.
Small GTPase Rah/Rab34 is associated with membrane ruffles and macropinosomes and promotes macropinosome formation.
J. Biol. Chem. 278, 4063-4071 (2003)
- Klein, C., Nguyen, D., Liu, C. H., Mizoguchi, A., Bhan, A. K., Miki, H., Takenawa, T., Rosen, F. S., Alt, F. W., Mulligan, R. C., and Snapper, S. B.
Gene therapy for Wiskott Aldrich Syndrome: rescue of T-cell signaling and amelioration of colitis upon transplantation of retrovirally transduced hematopoietic stem cells in mice.
Blood 101, 2159-2166 (2003)
- Nozumi, M., Nakagawa, H., Miki, H., Takenawa, T., and Miyamoto, S.
Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone.
J. Cell Sci. 116, 239-246 (2003)
- Abe, T., Kato, M., Miki, H., Takenawa, T., and Endo, T.
Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth.
J. Cell Sci. 116, 155-168 (2003)
- Suetsugu, S., Hattori, M., Miki, H., Tezuka, T., Yamamoto, T., Mikoshiba, K., and Takenawa, T.
Sustained Activation of N-WASP through Phosphorylation Is Essential for Neurite Extension.
Dev. Cell 3, 645-658 (2002).
- Yamaguchi, H., Miki, H., and Takenawa, T.
Two verprolin homology domains increase the Arp2/3 complex-mediated actin polymerization activities of N-WASP and WAVE1 C-terminal regions.
Biochem. Biophys. Res. Commun. 297, 214-219 (2002)
- Miki, H., and Takenawa, T.
WAVE2 serves a functional partner of IRSp53 by regulating its interaction with Rac.
Biochem. Biophys. Res. Commun. 293, 93-99 (2002)
- Yamaguchi, H., Miki, H., and Takenawa, T.
Neural Wiskott-Aldrich syndrome protein is involved in hepatocyte growth factor-induced migration, invasion, and tubulogenesis of epithelial cells.
Cancer Res. 62, 2503-2509 (2002)
- Suzuki, T., Mimuro, H., Suetsugu, S., Miki, H., Takenawa, T., and Sasakawa, C.
Neural Wiskott-Aldrich syndrome protein (N-WASP) is the specific ligand for Shigella VirG among the WASP family and determines the host cell type allowing actin-based spreading.
Cell. Microbiol. 4, 223-233 (2002)
- Suetsugu, S., Miki, H., and Takenawa, T.
Spatial and temporal regulation of actin polymerization for cytoskeleton formation through Arp2/3 complex and WASP/WAVE proteins.
Cell Motil. Cytoskeleton. 51, 113-122 (2002)
- Mizutani, K., Miki, H., He, H., Maruta, H., and Takenawa, T.
Essential role of neural Wiskott-Aldrich syndrome protein in podosome formation and degradation of extracellular matrix in src-transformed fibroblasts.
Cancer Res. 62, 669-674 (2002)
- Kato, M., Miki, H., Kurita, S., Endo, T., Nakagawa, H., Miyamoto, S., and Takenawa, T.
WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation.
Biochem. Biophys. Res. Commun. 291, 41-47 (2002)
- Vetterkind, S., Miki, H., Takenawa, T., Klawitz, I., Scheidtmann, K. H., and Preuss, U.
The rat homologue of Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) associates with actin filaments, recruits N-WASP from the nucleus, and mediates mobilization of actin from stress fibers in favor of filopodia formation.
J. Biol. Chem. 277, 87-95 (2002)
- Suetsugu, S., Miki, H., Yamaguchi, H., Obinata, T., and Takenawa, T.
Enhancement of branching efficiency by the actin filament-binding activity of N-WASP/WAVE2.
J. Cell Sci. 114, 4533-4542 (2001)
- Shcherbina, A., Miki, H., Kenney, D. M., Rosen, F. S., Takenawa, T., and Remold-O'Donnell, E.
WASP and N-WASP in human platelets differ in sensitivity to protease calpain.
Blood 98, 2988-2991 (2001)
- Suetsugu, S., Miki, H., and Takenawa, T
Identification of another Actin-related protein (Arp) 2/3 complex binding site in Neural-Aldrich syndrome protein (N-WASP), that complements actin polymerization induced by the Arp2/3 complex activating (VCA) domain of N-WASP.
J. Biol. Chem. 276, 33175-33180 (2001)
- Suetsugu, S., Miki, H., Yamaguchi, H., and Takenawa, T.
Requirement of the basic region of N-WASP/WAVE2 for actin-based motility.
Biochem. Biophys. Res. Commun. 282, 739-744 (2001)
- Takenawa, T., and Miki, H.
WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement.
J. Cell Sci. 114, 1801-1809 (2001)
- Martinez-Quiles, N., Rohatgi, R., Anton, I. M., Medina, M., Saville, P., Miki, H., Yamaguchi, H., Takenawa, T., Hartwig, J. H., Geha, R. S., and Ramesh, N.
WIP regulates N-WASP-mediated actin polymerization and filopodium formation.
Nat. Cell Biol. 3, 484-491 (2001)
- Nakagawa, H., Miki, H., Ito, M., Ohashi, K., Takenawa, T., and Miyamoto, S.
N-WASP, WAVE and Mena different roles in the organization of actin cytoskeleton in lamellipodia.
J. Cell Sci. 114, 1555-1565 (2001)
- Fukuoka, M., Suetsugu, S., Miki, H., Fukami, K., Endo, T., and Takenawa, T.
A Novel Neural Wiskott-Aldrich Syndrome Protein (N-WASP) Binding Protein, WISH, Induces Arp2/3 Complex Activation Independent of Cdc42.
J. Cell Biol. 152, 471-482 (2001)
- Miki, H., Yamaguchi, H., Suetsugu, S., and Takenawa, T
IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling.
Nature 408, 732-735 (2000)
- Yamaguchi, H., Miki, H., Suetsugu, S., Ma, L., Kirschner, M. W., and Takenawa, T.
Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP.
Proc. Natl. Acad. Sci. USA 97, 12631-12636 (2000)
- Yang, C., Huang, M., DeBiasio, J., Pring, M., Joyce, M., Miki, H., Takenawa, T., and Zigmond, S. H.
Profilin enhances Cdc42-induced nucleation of actin polymerization.
J. Cell Biol. 150, 1001-1012 (2000)
- Mimuro, H., Suzuki, T., Suetsugu, S., Miki, H., Ttakenawa, T. and Sasakawa C.
Profilin is required for sustaining efficient intra- and intercellular spreading of shigella flexneri.
J. Biol. Chem. 275, 28893-28901 (2000)
- Suzuki, T., Mimuro, H., Miki, H., Takenawa, T., Sasaki, T., Nakanishi, H., Takai, Y., and Sasakawa, C.
Rho Family GTPase Cdc42 Is Essential for the Actin-based Motility of Shigella in Mammalian Cells.
J. Exp. Med. 191, 1905-1920 (2000)
- Sasaki, N., Miki, H., and Takenawa, T
Arp2/3 Complex-Independent Actin Regulatory Function of WAVE.
Biochem. Biophys. Res. Commun. 272, 386-390 (2000)
- Banzai, Y., Miki, H., Yamaguchi, H., and Takenawa, T.
Essential role of neural Wiskott-Aldrich syndrome protein in neurite extension in PC12 cells and rat hippocampal primary culture cells.
J. Biol. Chem. 275, 11987-11992 (2000)
- Miki, H., Fukuda, M., Nishida, E., and Takenawa, T.
Phosphorylation of WAVE downstream of mitogen- activated protein kinase signaling.
J. Biol. Chem. 274, 27605-27609 (1999)
- Kato, M., Miki, H., Imai, K., Nonoyama, S., Suzuki, T., Sasakawa, C., and Takenawa, T.
Wiskott-Aldrich syndrome protein induces actin clustering without direct binding to Cdc42.
J. Biol. Chem. 274, 27225-27230 (1999)
- Suetsugu, S., Miki, H., and Takenawa, T.
Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis.
FEBS Lett. 457, 470-474 (1999)
- Imai, K., Nonoyama, S., Miki, H., Morio, T., Fukami, K., Zhu, Q., Aruffo, A., Ochs, H. D., Yata, J., and Takenawa, T.
The pleckstrin homology domain of the Wiskott-Aldrich syndrome protein is involved in the organization of actin cytoskeleton.
Clin. Immunol. 92, 128-137 (1999)
- Suetsugu, S., Miki, H., and Takenawa, T
Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex.
Biochem. Biophys. Res. Commun. 260, 296-302 (1999)
- Rohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., and Kirschner, M. W.
The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.
Cell 97, 221-231 (1999)
- Miki, H., Suetsugu, S., and Takenawa, T.
WAVE, a novel WASP-family protein involved in actin-reorganization induced by Rac.
EMBO J. 17, 6932-6941 (1998)
- Suetsugu, S., Miki, H., and Takenawa, T.
The essential role of profilin in the assembly of actin for microspike formation.
EMBO J. 17, 6516-6526 (1998)
- Suzuki, T., Miki, H., Takenawa, T., and Sasakawa, C.
Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.
EMBO J. 17, 2767-2776 (1998)
- Miki, H., and Takenawa, T.
Direct binding of verprolin-homology domain of N-WASP to actin, which is essential for cytoskeletal reorganization.
Biochem. Biophys. Res. Commun. 243, 73-78 (1998)
- Takenawa, T., Miki, H., and Matuoka, K.
Signaling through Grb2/Ash-Control of the Ras pathway and the cytoskeleton.
Curr. Top. Microbiol. Immunol. 228, 325-342 (1998)
- Miki, H., Sasaki, T., Takai, Y., and Takenawa, T.
Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP.
Nature 391, 93-96 (1998)
- Matuoka, K., Miki, H., Takahashi, K., and Takenawa, T.
A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs.
Biochem. Biophys. Res. Commun. 239, 488-492 (1997)
- Fukuoka, M., Miki, H., and Takenawa, T
Identification of N-WASP homologs in human and rat brain.
Gene 196, 43-48 (1997)
- Miki, H., Nonoyama, S., Zhu, Q., Aruffo, A., Ochs, H. D., Takenawa, T.
A tyrosine kinase signalling regulates WASP function, which is essential for megakaryocyte differentiation.
Cell Growth. Differ. 8, 195-202 (1997)
- Itoh, T., Miura, K., Miki, H., and Takenawa, T.
β-tubulin binds src homology 2 domains through a region different from the tyrosine-phosphorylated protein- recognizing site.
J. Biol. Chem. 271, 27931-27935 (1996)
- Miki, H., Miura, K., and Takenawa, T.
N-WASP, a novel actin-depolymerizing protein, regulates cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.
EMBO J. 15, 5326-5335 (1996)
- Takenawa, T., Miura, K., Miki, H., and Watanabe, K.
Signal transductions of SH2/SH3: Ash/Grb-2 downstream signaling.
Adv. in Pharmacol. 36, 139-153 (1996)
- Miura, K., Miki, H., Shimazaki, K., Kawai, N., and Takenawa, T.
Interaction of ash/Grb-2 via its SH3 domains with neuron-specific p150 and p65.
Biochem. J. 316, 639-645 (1996)
- Watanabe, K., Fukuchi, T., Hosoya, H., Shirasawa, T., Matuoka, K., Miki, H., and Takenawa, T.
Splicing isoforms of rat Ash/Grb2.
J. Biol. Chem. 270, 13733-13739 (1995)
- Nakanishi, H., Orita, S., Kaibuchi, K., Miura, K., Miki, H., Takenawa, T., and Takai, Y.
Kinetic properties of Ash/Grb2-interacting GDP/GTP exchange protein.
Biochem. Biophys. Res. Commun. 198, 1255-1261 (1994)
- Miki, H., Miura, K., Matuoka, K., Nakata, T., Hirokawa, N., Orita, S., Kaibuchi, K., Takai, Y., and Takenawa, T.
Association of Ash/Grb-2 with dynamin through the src homology 3 domain.
J. Biol. Chem. 269, 5489-5492 (1994)