/Genome Information Research Center  Next-Generation Sequencing Core Facility

  To prevent and control infectious diseases, it is essential to understand both the mechanisms of pathogenicity as well as host immune responses. The NGS Core Facility of the Genome Information Research Center was founded to support and provide genomic technologies for research on infectious diseases and immunology. We are supporting researchers in analyzing large volumes of data obtained from NGS and DNA microarrays by combining bioinformatics approaches with large computing systems designed for big data. Recently, we have begun supporting activities outside of infectious disease research for researchers from Osaka University as well as other universities.

Advanced NGS Instruments  

In the last decade, as a result of the remarkable technological innovation of NGS systems, which can read a massive number of sequences simultaneously and at high speed, we are now able to analyze genomic information quickly and at low cost. Various NGS instruments including IonPGM (Life Technologies), MiSeq, HiSeq (Illumina), and PacBio RSII (Pacific Biosciences) are available in our Core Facility. We provide genomics applications according to researchers’ needs in addition to training courses covering topics such as NGS procedures as well as other related experimental technologies. Furthermore, we are expanding research with the aim of improving bioinformatics analysis in collaboration with the Department of Genome Informatics and the Department of Infection Metagenomics.

  • Large-scale computer system for NGS

  • Next Generation Sequencer HiSeq

Staff

  • Head, Prof. : Sho Yamasaki (concur.)
  • SA Assoc. Prof. : Shota Nakamura (concur.)
  • SA Assoc. Prof. : Daisuke Okuzaki (concur.)
  • Asst. Prof.: Daisuke Motooka

Website

Publications

  • (1) Clinical implications of monitoring nivolumab immunokinetics in non-small cell lung cancer patients. Osa A., et al. JCI Insight (2018) Oct 4;3(19).
    (2) Heme ameliorates dextran sodium sulfate-induced colitis through
    providing intestinal macrophages with noninflammatory profiles. KayamaH., et al., Proc Natl Acad Sci U S A. (2018) Aug 14;115(33):8418-8423.
    (3) Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization. Kang S., et al., Nat Immunol. (2018) Jun;19(6):561-570.
    (4) Phenotype-genotype correlations of PIGO deficiency with variable phenotypes from infantile lethality to mild learning difficulties. Tanigawa J., et al., Hum Mutat. (2017) Mar 23. 38;7::805-815.