／Genome Information Research Center Next-Generation Sequencing Core Facility
To prevent and control infectious diseases, it is essential to understand both the mechanisms of pathogenicity as well as host immune responses. The NGS Core Facility of the Genome Information Research Center was founded to support and provide genomic technologies for research on infectious diseases and immunology. We are supporting researchers in analyzing large volumes of data obtained from NGS and DNA microarrays by combining bioinformatics approaches with large computing systems designed for big data. Recently, we have begun supporting activities outside of infectious disease research for researchers from Osaka University as well as other universities.
Advanced NGS Instruments
In the last decade, as a result of the remarkable technological innovation of NGS systems, which can read a massive number of sequences simultaneously and at high speed, we are now able to analyze genomic information quickly and at low cost. Various NGS instruments including IonPGM (Life Technologies), MiSeq, HiSeq (Illumina), and PacBio RSII (Pacific Biosciences) are available in our Core Facility. We provide genomics applications according to researchers’ needs in addition to training courses covering topics such as NGS procedures as well as other related experimental technologies. Furthermore, we are expanding research with the aim of improving bioinformatics analysis in collaboration with the Department of Genome Informatics and the Department of Infection Metagenomics.
- Head, Prof. : Sho Yamasaki (concur.)
- Assoc. Prof. : Shota Nakamura (concur.)
- SA Assoc. Prof.: Daisuke Okuzaki (concur.)
- Asst. Prof.: Daisuke Motooka
- (1) Direct RNA Sequencing Unfolds the Complex Transcriptome of Vibrio parahaemolyticus . Al Kadi M. et al. mSystems. 2021 Dec 21;6(6):e0099621
2) Identification of conserved SARS-CoV-2 spike epitopes that expand public cTfh clonotypes in mild COVID-19 patients. Lu X. et al. J Exp Med. 2021 Dec 6;218(12):e20211327
3) Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1-V2 and V3-V4 primer sets. Kameoka S. et al. BMC Genomics. 2021 Jul 10;22(1):527
4) Group 2 innate lymphoid cells support hematopoietic recovery under stress conditions . Sudo T. et al. J Exp Med. 2021 May 3;218(5):e20200817