Department of Experimental Genome Research

Staff:

Professor(SUP)

Masahito Ikawa, Ph.D.

Lecturer(SUP)

Yuhkoh Satouh, Ph.D.

Assistant Professor

Yoshitaka Fujihara, Ph.D.

Haruhiko Miyata, Ph.D.

Taichi Noda, Ph.D.

Specially Appointed Assistant Professor(SUP)

Daiji Kiyozumi, Ph.D.

Tsutomu Endo, Ph.D.

Specially Appointed Assistant Professor

Julio Manuel Castaneda, Ph.D.

Specially Appointed Researcher

Kanako Kita, Ph.D.

Kaori Nozawa, D.V.M., Ph.D.

Keisuke Shimada, D.V.M., Ph.D

Nobuyuki Sakurai, Ph.D.

Yonggang Lu, Ph.D.

Technical Staff

Naoko Nagasawa

Graduate Student

Asami Oji

Tomohiro Tobita

Takafumi Matsumura

Akane Morohoshi

Soojin Park

Jiang Sun

Ferheen Abbasi

Seiya Oura

Undergraduate Student

Kiyonori Kobayashi

Tamara Larasati

Kentaro Shimada

Mayo Kodani

Research Student

Xu Zoulan

Guest Professor

Martin M. Matzuk

Guest Associate Professor

Ayako Isotani

Subjects:

1. Analysis of molecular mechanisms involved in mammalian gametogenesis, fertilization, and implantation.
2. Development of new technologies to produce genetically manipulated animals. (CRISPR/Cas system, lentiviral vectors, etc.).

Papers published in 2017

1. A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.

   Ayako Isotani, Takafumi Matsumura, Masaki Ogawa, Takahiro Tanaka, Kazuo Yamagata, Masahito Ikawa and Masaru Okabe

   Biol Reprod, 97 (1), 61-68, 2017.
2. Suppression of HBV replication by the expression of nickase- and nuclease dead-Cas9.

   Takeshi Kurihara, Takasuke Fukuhara, Chikako Ono, Satomi Yamamoto, Kentaro Uemura, Toru Okamoto, Masaya Sugiyama, Daisuke Motooka, Shota Nakamura, Masahito Ikawa, Masashi Mizokami, Yoshihiko Maehara and Yoshiharu Matsuura

   Sci Rep, 7 (1), 6122, 2017.
3. Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models.

   Yoshitaka Fujihara, Asami Oji, Tamara Larasati, Kanako Kojima-Kita and Masahito Ikawa

   Int J Mol Sci, 18 (10), 2208, 2017.
4. Mouse spermatozoa with higher fertilization rates have thinner nuclei.

   Daisuke Mashiko, Masahito Ikawa and Koichi Fujimoto

   PeerJ, 5, e3913, 2017.
5. Placenta-specific gene manipulation using lentiviral vector and its application.

   Tomohiro Tobita, Daiji Kiyozumi and Masahito Ikawa

   Placenta, 59 Suppl 1, S37-S43, 2017.
6. Deletion of PIKfyve alters alveolar macrophage populations and exacerbates allergic inflammation in mice.

   Takumi Kawasaki, Kosuke Ito, Haruhiko Miyata, Shizuo Akira and Taro Kawai

   EMBO J, 36 (12), 1707-1718, 2017.
7. TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa.

   Julio M Castaneda, Rong Hua, Haruhiko Miyata, Asami Oji, Yueshuai Guo, Yiwei Cheng, Tao Zhou, Xuejiang Guo, Yiqiang Cui, Bin Shen, Zibin Wang, Zhibin Hu, Zuomin Zhou, Jiahao Sha, Renata Prunskaite-Hyyrylainen, Zhifeng Yu, Ramiro Ramirez-Solis, Masahito Ikawa, Martin M Matzuk and Mingxi Liu

   Proc Natl Acad Sci U S A, 114 (27), E5370-E5378, 2017.
8. The mechanics clarifying counterclockwise rotation in most IVF eggs in mice.

   Kenta Ishimoto, Masahito Ikawa and Masaru Okabe

   Sci Rep, 7, 43456, 2017.
9. Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.

   Takuya Ishikawa, Yasuyuki Kitaura, Yoshihiro Kadota, Yukako Morishita, Miki Ota, Fumiya Yamanaka, Minjun Xu, Masahito Ikawa, Naokazu Inoue, Fuminori Kawano, Naoya Nakai, Taro Murakami, Shinji Miura, Yukino Hatazawa, Yasutomi Kamei and Yoshiharu Shimomura

   Sci Rep, 7, 39825, 2017.
10. Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis.

    Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka and Kazuo Yamagata

    Cell Rep, 18 (3), 593-600, 2017.
11. Proton Pump Inhibitors Decrease Soluble fms-Like Tyrosine Kinase-1 and Soluble Endoglin Secretion, Decrease Hypertension, and Rescue Endothelial Dysfunction.

    Kenji Onda, Stephen Tong, Sally Beard, Natalie Binder, Masanaga Muto, Sevvandi N Senadheera, Laura Parry, Mark Dilworth, Lewis Renshall, Fiona Brownfoot, Roxanne Hastie, Laura Tuohey, Kirsten Palmer, Toshihiko Hirano, Masahito Ikawa, Tu'uhevaha Kaitu'u-Lino and Natalie J Hannan

    Hypertension, 69 (3), 457-468, 2017.
12. Viable offspring after imaging of Ca2+ oscillations and visualization of the cortical reaction in mouse eggs†.

    Yuhkoh Satouh, Kaori Nozawa, Kazuo Yamagata, Takao Fujimoto and Masahito Ikawa

    Biol Reprod, 96 (3), 563-575, 2017.
13. New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice.

    Chie Naruse, Shinwa Shibata, Masaru Tamura, Takayuki Kawaguchi, Kanae Abe, Kazushi Sugihara, Tomoaki Kato, Takumi Nishiuchi, Shigeharu Wakana, Masahito Ikawa and Masahide Asano

    FASEB J, 31 (6), 2252-2266, 2017.
14. A chromosome 16p13.11 microduplication causes hyperactivity through dysregulation of miR-484/protocadherin-19 signaling.

    M Fujitani, S Zhang, R Fujiki, Y Fujihara and T Yamashita

    Mol Psychiatry, 22 (3), 364-374, 2017.

Link

Department of Experimental Genome Research

Animal Resource Center for Infectious Diseases

Research Institute for Microbial Diseases, Osaka University