Department of Virology

Staff:

Professor

Kazuyoshi Ikuta, Ph.D.

Assistant Professer

Takeshi Kurosu, D.V.M., Ph.D.

Yohei Watanabe, D.V.M., Ph.D.

Specially Appointed Associate Professor

Tadahiro Sasaki, Ph.D.

Specially Appointed Researcher

Yuji Inoue, Ph.D.

Mitsuhiro Nishimura, Ph.D.

Collaborator

Ritsuko Koketsu, Ph.D.

Graduate Student

Omokoko Mogot Diata

Sabar Pambudi

Supranee Phanthanawiboon

Ramadhany Ririn

Yan Pan

Yasuha Arai

Priya Dhole

Yuka Ohori

Keiko Hanabara

Subjects:

1. Characterization of H5N1? influenza virus variants with hemagglutinin mutations isolated in patients
2. Characterization of human monoclonal antibodies that broadly cross-neutralize influenza A viruses
3. Development of mouse model for dengue virus infection.
4. Development of protease inhibitor against dengue virus
5. Characterization of human monoclonal antibodies that broadly cross-neutralize dengue viruses

Papers published in 2013

1. Variables influencing anti-human immunodeficiency virus type 1 neutralizing human monoclonal antibody (NhMAb) production among infected Thais.

   Siriwat Akapirat, Anchalee Avihingsanon, Jintanat Ananworanich, Alexandra Schuetz, Pongrama Ramasoota, Natthanej Luplertlop, Ken-Ichiro Ono, Kazuyoshi Ikuta, Piraporn Utachee, Masanori Kameoka and Pornsawan Leaungwutiwong

   Southeast Asian J Trop Med Public Health, 44 (5), 825-841, 2013.
2. Antiviral Protein of Momordica charantia L. Inhibits Different Subtypes of Influenza A.

   Viroj Pongthanapisith, Kazuyoshi Ikuta, Pilaipan Puthavathana and Wichet Leelamanit

   Evid Based Complement Alternat Med, 2013, 729081, 2013.
3. Characterization of chikungunya virus infection of a human keratinocyte cell line: role of mosquito salivary gland protein in suppressing the host immune response.

   Orapim Puiprom, Ronald Enrique Morales Vargas, Rutcharin Potiwat, Panjaporn Chaichana, Kazuyoshi Ikuta, Pongrama Ramasoota and Tamaki Okabayashi

   Infect Genet Evol, 17, 210-215, 2013.
4. Detection and characterization of enteric viruses in flood water from the 2011 thai flood.

   Nathamon Ngaosuwankul, Narin Thippornchai, Akifumi Yamashita, Ronald E Morales Vargas, Witawat Tunyong, Yuvadee Mahakunkijchareon, Kazuyoshi Ikuta, Pratap Singhasivanon, Tamaki Okabayashi and Pornsawan Leaungwutiwong

   Jpn J Infect Dis, 66 (5), 398-403, 2013.
5. Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein.

   Azumi Hirata, Yuki Hori, Yuichi Koga, Jun Okada, Akikazu Sakudo, Kazuyoshi Ikuta, Shigenori Kanaya and Kazufumi Takano

   BMC Biotechnol, 13, 19, 2013.
6. Jurkat cell proliferation is suppressed by Chlamydia (Chlamydophila) pneumoniae infection accompanied with attenuation of phosphorylation at Thr389 of host cellular p70S6K.

   Itaru Hirai, Megumi Ebara, Shoko Nakanishi, Chihiro Yamamoto, Tadahiro Sasaki, Kazuyoshi Ikuta and Yoshimasa Yamamoto

   Immunobiology, 218 (4), 527-532, 2013.
7. Misinterpretation in virus clearance studies of biological products due to an uncommon discrepancy between cytopathic effects and infectivity of human immunodeficiency virus (HIV).

   Takeru Urayama, Roslyn Cameron, Tetsuo Sato, Mikihiro Yunoki and Kazuyoshi Ikuta

   Biologicals, 41 (2), 125-127, 2013.
8. Discovery of novel low-molecular-weight HIV-1 inhibitors interacting with cyclophilin A using in silico screening and biological evaluations.

   Yu-Shi Tian, Chris Verathamjamras, Norihito Kawashita, Kousuke Okamoto, Teruo Yasunaga, Kazuyoshi Ikuta, Masanori Kameoka and Tatsuya Takagi

   J Mol Model, 19 (1), 465-475, 2013.
9. Chikungunya virus induces a more moderate cytopathic effect in mosquito cells than in mammalian cells.

   Yong-Gang Li, Uamporn Siripanyaphinyo, Uranan Tumkosit, Nitchakarn Noranate, Atchareeya A-nuegoonpipat, Ran Tao, Takeshi Kurosu, Kazuyoshi Ikuta, Naokazu Takeda and Surapee Anantapreecha

   Intervirology, 56 (1), 6-12, 2013.
10. Emerging antigenic variants at the antigenic site Sb in pandemic A(H1N1)2009 influenza virus in Japan detected by a human monoclonal antibody.

    Mayo Yasugi, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Norihito Kawashita, Anariwa Du, Ryo Misaki, Motoki Kuhara, Naphatsawan Boonsathorn, Kazuhito Fujiyama, Yoshinobu Okuno, Takaaki Nakaya and Kazuyoshi Ikuta

    PLoS One, 8 (10), e77892, 2013.
11. Immunization of rabbits with synthetic peptides derived from a highly conserved β-sheet epitope region underneath the receptor binding site of influenza A virus.

    Shoji Ideno, Kaoru Sakai, Mikihiro Yunoki, Ritsuko Kubota-Koketsu, Yuji Inoue, Shota Nakamura, Teruo Yasunaga, Yoshinobu Okuno and Kazuyoshi Ikuta

    Biologics, 7, 233-241, 2013.
12. N 2 gas plasma inactivates influenza virus by inducing changes in viral surface morphology, protein, and genomic RNA.

    Akikazu Sakudo, Naohiro Shimizu, Yuichiro Imanishi and Kazuyoshi Ikuta

    Biomed Res Int, 2013, 694269, 2013.
13. A small compound targeting the interaction between nonstructural proteins 2B and 3 inhibits dengue virus replication.

    Sabar Pambudi, Norihito Kawashita, Supranee Phanthanawiboon, Magot Diata Omokoko, Promsin Masrinoul, Akifumi Yamashita, Kriengsak Limkittikul, Teruo Yasunaga, Tatsuya Takagi, Kazuyoshi Ikuta and Takeshi Kurosu

    Biochem Biophys Res Commun, 440 (3), 393-398, 2013.
14. Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus.

    Chonlatip Pipattanaboon, Tadahiro Sasaki, Mitsuhiro Nishimura, Chayanee Setthapramote, Pannamthip Pitaksajjakul, Pornsawan Leaungwutiwong, Kriengsak Limkittikul, Orapim Puiprom, Mikiko Sasayama, Panjaporn Chaichana, Tamaki Okabayashi, Takeshi Kurosu, Ken-Ichiro Ono, Pongrama Ramasoota and Kazuyoshi Ikuta

    Biologics, 7, 175-187, 2013.
15. Serotype-specific anti-Dengue virus NS1 mouse antibodies cross-react with prM and are potentially involved in virus production.

    Promsin Masrinoul, Magot Diata Omokoko, Sabar Pambudi, Kazuyoshi Ikuta and Takeshi Kurosu

    Viral Immunol, 26 (4), 250-258, 2013.
16. Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.

    Tadahiro Sasaki, Chayanee Setthapramote, Takeshi Kurosu, Mitsuhiro Nishimura, Azusa Asai, Magot D Omokoko, Chonlatip Pipattanaboon, Pannamthip Pitaksajjakul, Kriengsak Limkittikul, Arunee Subchareon, Panjaporn Chaichana, Tamaki Okabayashi, Itaru Hirai, Pornsawan Leaungwutiwong, Ryo Misaki, Kazuhito Fujiyama, Ken-Ichiro Ono, Yoshinobu Okuno, Pongrama Ramasoota and Kazuyoshi Ikuta

    Antiviral Res, 98 (3), 423-431, 2013.
17. Human monoclonal antibodies broadly neutralizing against influenza B virus.

    Mayo Yasugi, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Norihito Kawashita, Anariwa Du, Tadahiro Sasaki, Mitsuhiro Nishimura, Ryo Misaki, Motoki Kuhara, Naphatsawan Boonsathorn, Kazuhito Fujiyama, Yoshinobu Okuno, Takaaki Nakaya and Kazuyoshi Ikuta

    PLoS Pathog, 9 (2), e1003150, 2013.
18. Induction of anti-influenza immunity by modified green fluorescent protein (GFP) carrying hemagglutinin-derived epitope structure.

    Yuji Inoue, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Mitsuhiro Nishimura, Shoji Ideno, Ken-ichiro Ono, Yoshinobu Okuno and Kazuyoshi Ikuta

    J Biol Chem, 288 (7), 4981-4990, 2013.
19. Evolution and control of H5N1. A better understanding of the evolution and diversity of H5N1 flu virus and its host species in endemic areas could inform more efficient vaccination and control strategies.

    Yohei Watanabe, Madiha S Ibrahim and Kazuyoshi Ikuta

    EMBO Rep, 14 (2), 117-122, 2013.

Link

http://www.biken.osaka-u.ac.jp/