Department of Virology

Subjects:

1. Epidemiological, virological and molecular-biological characterization of influenza virus and dengue virus.
2. Therapeutics and diagnostics using monoclonal antibodies against influenza virus and dengue virus.
3. Development of peptide-based boost vaccine against influenza and dengue.
4. Biosafety of blood products from infectious agents.

Published Papers

1. Immunofluorescence assay in India for confirmation of HIV-1 infection using a T-cell line infected with defective HIV-1.

   Manju Bala, Juan F Arias, Monorama Deb and Kazuyoshi Ikuta

   Int J Infect Dis, 14 (12), e1093-1098, 2010.
2. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.

   Hiroyuki Kayamuro, Yasuo Yoshioka, Yasuhiro Abe, Shuhei Arita, Kazufumi Katayama, Tetsuya Nomura, Tomoaki Yoshikawa, Ritsuko Kubota-Koketsu, Kazuyoshi Ikuta, Shigefumi Okamoto, Yasuko Mori, Jun Kunisawa, Hiroshi Kiyono, Norio Itoh, Kazuya Nagano, Haruhiko Kamada, Yasuo Tsutsumi and Shin-ichi Tsunoda

   J Virol, 84 (24), 12703-12712, 2010.
3. Appearance of Drug Resistance-Associated Mutations in Human Immunodeficiency Virus Type 1 CRF01_AE Integrase Derived from Drug-Naive Thai Patients.

   Panasda Isarangkura-Na-Ayuthaya, Wiyada Kaewnoo, Wattana Auwanit, U Chandimal de Silva, Kazuyoshi Ikuta, Pathom Sawanpanyalert and Masanori Kameoka

   AIDS Res Hum Retroviruses, 26 (12), 1341-1343, 2010.
4. Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones.

   Yuta Kanai, Malinee Chittaganpitch, Izuru Nakamura, Gui-Mei Li, Gui-Rong Bai, Yong-Gang Li, Kazuyoshi Ikuta and Pathom Sawanpanyalert

   Virus Res, 153 (2), 218-225, 2010.
5. Design and evaluation of antiretroviral peptides corresponding to the C-terminal heptad repeat region (C-HR) of human immunodeficiency virus type 1 envelope glycoprotein gp41.

   Bongkot Soonthornsata, Yu-Shi Tian, Piraporn Utachee, Sompong Sapsutthipas, Panasda Isarangkura-na-Ayuthaya, Wattana Auwanit, Tatsuya Takagi, Kazuyoshi Ikuta, Pathom Sawanpanyalert, Norihito Kawashita and Masanori Kameoka

   Virology, 405 (1), 157-164, 2010.
6. Sustained appearance of drug resistance-associated mutations in HIV-1 CRF01_AE protease and reverse transcriptase derived from protease inhibitor-naive Thai patients.

   Duangrat Jullaksorn, Samatchaya Boonchawalit, Jiraporn Uttiyoung, Bongkot Soonthornsata, Amara Yowang, Nongkran Krathong, Sununta Chautrakul, Kazuyoshi Ikuta, Amornsak Roobsoong, Sangkom Kanitvittaya, Pathom Sawanpanyalert and Masanori Kameoka

   Southeast Asian J Trop Med Public Health, 41 (2), 347-357, 2010.
7. Design and evaluation of antiretroviral peptides corresponding to the C-terminal heptad repeat region (C-HR) of human immunodeficiency virus type 1 envelope glycoprotein gp41.

   Bongkot Soonthornsata, Yu-Shi Tian, Piraporn Utachee, Sompong Sapsutthipas, Panasda Isarangkura-na-Ayuthaya, Wattana Auwanit, Tatsuya Takagi, Kazuyoshi Ikuta, Pathom Sawanpanyalert, Norihito Kawashita and Masanori Kameoka

   Virology, 405 (1), 157-164, 2010.
8. Fab MAbs specific to HA of influenza virus with H5N1 neutralizing activity selected from immunized chicken phage library.

   Pannamthip Pitaksajjakul, Porntippa Lekcharoensuk, Narin Upragarin, Carlos F Barbas, Madiha Salah Ibrahim, Kazuyoshi Ikuta and Pongrama Ramasoota

   Biochem Biophys Res Commun, 395 (4), 496-501, 2010.
9. The impact of antigenic drift of influenza A virus on human herd immunity: Sero-epidemiological study of H1N1 in healthy Thai population in 2009.

   Yuta Kanai, Naphatsawan Boonsathorn, Malinee Chittaganpitch, Guirong Bai, Yonggang Li, Tetsuo Kase, Kazuo Takahashi, Yoshinobu Okuno, Wipawee Jampangern, Kazuyoshi Ikuta and Pathom Sawanpanyalert

   Vaccine, 28 (33), 5437-5444, 2010.
10. The role of lysine residue at amino acid position 165 of human immunodeficiency virus type 1 CRF01_AE Gag in reducing viral drug susceptibility to protease inhibitors.

    Masanori Kameoka, Panasda Isarangkura-na-Ayuthaya, Yoko Kameoka, Sompong Sapsutthipas, Bongkot Soonthornsata, Shota Nakamura, Kenzo Tokunaga, Pathom Sawanpanyalert, Kazuyoshi Ikuta and Wattana Auwanit

    Virology, 405 (1), 129-138, 2010.
11. Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies.

    Yuta Kanai, Muneo Tsujikawa, Mikihiro Yunoki, Shoko Nishiyama, Kazuyoshi Ikuta and Katsuro Hagiwara

    J Med Virol, 82 (1), 69-76, 2010.
12. Characterization of H5N1 influenza viruses isolated from humans in vitro.

    Yong-Gang Li, Malinee Chittaganpitch, Sunthareeya Waicharoen, Yuta Kanai, Gui-Rong Bai, Masanori Kameoka, Naokazu Takeda, Kazuyoshi Ikuta and Pathom Sawanpanyalert

    Virol J, 7, 112, 2010.
13. Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows.

    Myra Hosmillo, Young-Ju Jeong, Hyun-Jeong Kim, Jun-Gyu Park, Mukti Kant Nayak, Mia Madel Alfajaro, Therese Marie Collantes, Su-Jin Park, Kazuyoshi Ikuta, Mikihiro Yunoki, Mun-Il Kang, Sang-Ik Park and Kyoung-Oh Cho

    Arch Virol, 155 (7), 1157-1161, 2010.
14. Full-Length Sequences of One Genotype 4 and Three Genotype 3 Hepatitis E Viruses in Fecal Samples from Domestic Swine in Japan.

    Takeru Urayama, Sompong Sapsutthipas, Muneo Tsujikawa, Akifumi Yamashita, Hiromi Nishigaki, Madiha S. Ibrahim, Katsuro Hagiwara, Mikihiro Yunoki, Teruo Yasunaga, Teruhide Yamaguchi and Kazuyoshi Ikuta

    Open Vet Sci J, 4, 11-19, 2010.
15. Highly efficient rescue of dengue virus using a co-culture system with mosquito/mammalian cells.

    Takeshi Kurosu, Chidchanok Khamlert, Supranee Phanthanawiboon, Kazuyoshi Ikuta and Surapee Anantapreecha

    Biochem Biophys Res Commun, 394 (2), 398-404, 2010.
16. High systemic levels of interleukin-10, interleukin-22 and C-reactive protein in Indian patients are associated with low in vitro replication of HIV-1 subtype C viruses.

    Juan F Arias, Reiko Nishihara, Manju Bala and Kazuyoshi Ikuta

    Retrovirology, 7, 15, 2010.
17. Two N-linked glycosylation sites in V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 regulate viral neutralization susceptibility to the human monoclonal antibody specific for CD4 binding domain.

    Piraporn Utachee, Shota Nakamura, Panasda Isarangkura-Na-Ayuthaya, Kenzo Tokunaga, Pathom Sawanpanyalert, Kazuyoshi Ikuta, Wattana Auwanit and Masanori Kameoka

    J Virol, 84 (9), 4311-4320, 2010.
18. Highly conserved sequences for human neutralization epitope on hemagglutinin of influenza A viruses H3N2, H1N1 and H5N1: Implication for human monoclonal antibody recognition.

    Akifumi Yamashita, Norihito Kawashita, Ritsuko Kubota-Koketsu, Yuji Inoue, Yohei Watanabe, Madiha S Ibrahim, Shoji Ideno, Mikihiro Yunoki, Yoshinobu Okuno, Tatsuya Takagi, Teruo Yasunaga and Kazuyoshi Ikuta

    Biochem Biophys Res Commun, 393 (4), 614-618, 2010.
19. Endogenous non-retroviral RNA virus elements in mammalian genomes.

    Masayuki Horie, Tomoyuki Honda, Yoshiyuki Suzuki, Yuki Kobayashi, Takuji Daito, Tatsuo Oshida, Kazuyoshi Ikuta, Patric Jern, Takashi Gojobori, John M Coffin and Keizo Tomonaga

    Nature, 463 (7277), 84-87, 2010.
20. Highly Pathogenic H5N1 Avian Influenza Virus Induces Extracellular Ca2+ Influx, Leading to Apoptosis in Avian Cells.

    Mayo Ueda, Tomo Daidoji, Anariwa Du, Cheng-Song Yang, Madiha S Ibrahim, Kazuyoshi Ikuta and Takaaki Nakaya

    J Virol, 84 (6), 3068-3078, 2010.
21. Structure of the Prion Protein and its Gene: An Analysis using Bioinformatics and Computer Simulation.

    Akikazu Sakudo, Guangai Xue, Norihito Kawashita, Yasuhisa Ano, Tatsuya Takagi, Hideharu Shintani, Yasuharu Tanaka, Takashi Onodera and Kazuyoshi Ikuta

    Curr Protein Pept Sci, 11 (2), 166-179, 2010.
22. Infectious prion protein in the filtrate even after 15nm filtration.

    Mikihiro Yunoki, Hiroyuki Tanaka, Takeru Urayama, Yuta Kanai, Aya Nishida, Maki Yoshikawa, Yuji Ohkubo, Yoshiyasu Kawabata, Katsuro Hagiwara and Kazuyoshi Ikuta

    Biologicals, 38 (2), 311-313, 2010.
23. Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies.

    Yuta Kanai, Muneo Tsujikawa, Mikihiro Yunoki, Shoko Nishiyama, Kazuyoshi Ikuta and Katsuro Hagiwara

    J Med Virol, 82 (1), 69-76, 2010.
24. Genotypic Characterization of HIV Type 1 env gp160 Sequences from Three Regions in Thailand.

    U Chandimal de Silva, Jiranan Warachit, Nerisa Sattagowit, Chanin Jirapongwattana, Sumolrat Panthong, Piraporn Utachee, Teruo Yasunaga, Kazuyoshi Ikuta, Masanori Kameoka and Naphatsawan Boonsathorn

    AIDS Res Hum Retroviruses, 26 (2), 223-227, 2010.
25. Structure of the Prion Protein and its Gene: An Analysis using Bioinformatics and Computer Simulation.

    Akikazu Sakudo, Guangai Xue, Norihito Kawashita, Yasuhisa Ano, Tatsuya Takagi, Hideharu Shintani, Yasuharu Tanaka, Takashi Onodera and Kazuyoshi Ikuta

    Curr Protein Pept Sci, 11 (2), 166-179, 2010.
26. Degradation and destabilization of abnormal prion protein using alkaline detergents and proteases.

    Yoshihiko Hirata, Hitoshi Ito, Taro Furuta, Kazuyoshi Ikuta and Akikazu Sakudo

    Int J Mol Med, 25 (2), 267-270, 2010.
27. Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1.

    Mikihiro Yunoki, Ritsuko Kubota-Koketsu, Takeru Urayama, Tadahiro Sasaki, Du Analiwa, Yuko Konoshima, Shoji Ideno, Yuki Fukunaga, Saeko Morikawa, Satoshi Hiroi, Kazuo Takahashi, Yoshinobu Okuno, Katsuro Hagiwara and Kazuyoshi Ikuta

    Br J Haematol, 148 (6), 953-955, 2010.

Link

http://www.biken.osaka-u.ac.jp/