Department of Experimental Genome Research

Subjects:

1. Analysis of Molecular mechanisms involved in reproduction using genetically modified animals.
2. Development of new technologies to produce genetically manipulated animals by using Lentiviral vectors and RNA interference.
3. Analysis of sex differentiation of germ cells and preimplantation embryos.
4. Investigation of the molecular biological mechanisms involved in cardiovascular diseases.

Published Papers

1. The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.

   Hiroyuki Oshiumi, Moeko Miyashita, Naokazu Inoue, Masaru Okabe, Misako Matsumoto and Tsukasa Seya

   Cell Host Microbe, 8 (6), 496-509, 2010.
2. Novel method of gene transfer in birds: intracytoplasmic sperm injection for green fluorescent protein expression in quail blastoderms.

   Shusei Mizushima, Soichi Takagi, Tamao Ono, Yusuke Atsumi, Akira Tsukada, Noboru Saito, Tomohiro Sasanami, Masaru Okabe and Kiyoshi Shimada

   Biol Reprod, 83 (6), 965-969, 2010.
3. Establishment and analysis of SLC22A12 (URAT1) knockout mouse.

   M Hosoyamada, Y Takiue, H Morisaki, J Cheng, M Ikawa, M Okabe, T Morisaki, K Ichida, T Hosoya and T Shibasaki

   Nucleosides Nucleotides Nucleic Acids, 29 (4-6), 314-320, 2010.
4. Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse.

   Naokazu Inoue, Tatsuya Kasahara, Masahito Ikawa and Masaru Okabe

   PLoS One, 5 (4), e10301, 2010.
5. Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse.

   Yoshitaka Fujihara, Masao Murakami, Naokazu Inoue, Yuhkoh Satouh, Kazuhiro Kaseda, Masahito Ikawa and Masaru Okabe

   J Cell Sci, 123 (Pt 9), 1531-1536, 2010.
6. Fertilization: a sperm's journey to and interaction with the oocyte.

   Masahito Ikawa, Naokazu Inoue, Adam M Benham and Masaru Okabe

   J Clin Invest, 120 (4), 984-994, 2010.
7. Expression of complement regulatory protein on porcine endogenous retrovirus (PERV) depends on molecular size.

   Aki Yamamoto, Chizuko Kobayashi, Saya Yamashita, Takayuki Miyazawa, Masaru Okabe, Masahiro Fukuzawa and Shuji Miyagawa

   Transpl Immunol, 23 (1-2), 71-76, 2010.
8. Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.

   Hidetoshi Hasuwa, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto and Masaru Okabe

   Exp Anim, 59 (1), 105-107, 2010.
9. Humanized gene replacement in mice reveals the contribution of cancer stroma-derived HB-EGF to tumor growth.

   Tomoko Ichise, Satoshi Adachi, Minako Ohishi, Masahito Ikawa, Masaru Okabe, Ryo Iwamoto and Eisuke Mekada

   Cell Struct Funct, 35 (1), 3-13, 2010.
10. The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos.

    Shin Kobayashi, Yoshitaka Fujihara, Nathan Mise, Kazuhiro Kaseda, Kuniya Abe, Fumitoshi Ishino and Masaru Okabe

    Nucleic Acids Res, 38 (11), 3672-3681, 2010.
11. Survey of glycoantigens in cells from alpha1-3galactosyltransferase knockout pig using a lectin microarray.

    Shuji Miyagawa, Shunsaku Takeishi, Aki Yamamoto, Kosuke Ikeda, Hitomi Matsunari, Masao Yamada, Masaru Okabe, Eiji Miyoshi, Masahiro Fukuzawa and Hiroshi Nagashima

    Xenotransplantation, 17 (1), 61-70, 2010.
12. Complement regulation in the GalT KO era.

    Shuji Miyagawa, Aki Yamamoto, Katsuyoshi Matsunami, Dandan Wang, Yuichi Takama, Takehisa Ueno, Masaru Okabe, Hiroshi Nagashima and Masahiro Fukuzawa

    Xenotransplantation, 17 (1), 11-25, 2010.
13. A newly cloned pig dolichyl-phosphate mannosyl-transferase for preventing the transmission of porcine endogenous retrovirus to human cells.

    Aki Yamamoto, Shino Nakatsu, Akihiro Kondo, Tetsuya Asato, Masaru Okabe, Masahiro Fukuzawa and Shuji Miyagawa

    Transpl Int, 23 (4), 424-431, 2010.

Link

http://www.biken.osaka-u.ac.jp/