Department of Experimental Genome Research

Subjects:

1. Analysis of Molecular mechanisms involved in reproduction using genetically modified animals.
2. Development of new technologies to produce genetically manipulated animals by using Lentiviral vectors and RNA interference.
3. Analysis of sex differentiation of germ cells and preimplantation embryos.
4. Investigation of the molecular biological mechanisms involved in cardiovascular diseases.

Published Papers

1. Placenta-specific gene activation and inactivation using integrase-defective lentiviral vectors with the Cre/LoxP system.

   Yuka Morioka, Ayako Isotani, Robert G Oshima, Masaru Okabe and Masahito Ikawa

   Genesis, 47 (12), 793-798, 2009.
2. Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis.

   Atsushi Saito, Shin-ichiro Hino, Tomohiko Murakami, Soshi Kanemoto, Shinichi Kondo, Masahiro Saitoh, Riko Nishimura, Toshiyuki Yoneda, Tatsuya Furuichi, Shiro Ikegawa, Masahito Ikawa, Masaru Okabe and Kazunori Imaizumi

   Nat Cell Biol, 11 (10), 1197-1204, 2009.
3. Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation.

   Tomohiko Murakami, Atsushi Saito, Shin-ichiro Hino, Shinichi Kondo, Soshi Kanemoto, Kazuyasu Chihara, Hiroshi Sekiya, Kenji Tsumagari, Kimiko Ochiai, Kazuya Yoshinaga, Masahiro Saitoh, Riko Nishimura, Toshiyuki Yoneda, Ikuyo Kou, Tatsuya Furuichi, Shiro Ikegawa, Masahito Ikawa, Masaru Okabe, Akio Wanaka and Kazunori Imaizumi

   Nat Cell Biol, 11 (10), 1205-1211, 2009.
4. A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice.

   Chikara Kokubu, Kyoji Horie, Koichiro Abe, Ryuji Ikeda, Sumi Mizuno, Yoshihiro Uno, Sanae Ogiwara, Masato Ohtsuka, Ayako Isotani, Masaru Okabe, Kenji Imai and Junji Takeda

   Nat Genet, 41 (8), 946-952, 2009.
5. A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf-Hirschhorn syndrome.

   Keisuke Nimura, Kiyoe Ura, Hidetaka Shiratori, Masato Ikawa, Masaru Okabe, Robert J Schwartz and Yasufumi Kaneda

   Nature, 460 (7252), 287-291, 2009.
6. Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse.

   Ryo Yamaguchi, Yuko Muro, Ayako Isotani, Keizo Tokuhiro, Kazuhiro Takumi, Ibrahim Adham, Masahito Ikawa and Masaru Okabe

   Biol Reprod, 81 (1), 142-146, 2009.
7. L-amino acid oxidase plays a crucial role in host defense in the mammary glands.

   Kentaro Nagaoka, Fugaku Aoki, Mizuna Hayashi, Yoshikage Muroi, Toshihiro Sakurai, Kikuji Itoh, Masahito Ikawa, Masaru Okabe, Kazuhiko Imakawa and Senkiti Sakai

   FASEB J, 23 (8), 2514-2520, 2009.
8. Targeted gene modification in mouse ES cells using integrase-defective lentiviral vectors.

   Yuka Okada, Yuko Ueshin, Hidetoshi Hasuwa, Kazuhiro Takumi, Masaru Okabe and Masahito Ikawa

   Genesis, 47 (4), 217-223, 2009.
9. Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration.

   Makoto Nishizuka, Keishi Kishimoto, Ayumi Kato, Masahito Ikawa, Masaru Okabe, Ryuichiro Sato, Hiroyuki Niida, Makoto Nakanishi, Shigehiro Osada and Masayoshi Imagawa

   Exp Cell Res, 315 (5), 809-819, 2009.
10. Peroxiredoxin 4 knockout results in elevated spermatogenic cell death via oxidative stress.

    Yoshihito Iuchi, Futoshi Okada, Satoshi Tsunoda, Noriko Kibe, Nobuyuki Shirasawa, Masahito Ikawa, Masaru Okabe, Yoshitaka Ikeda and Junichi Fujii

    Biochem J, 419 (1), 149-158, 2009.
11. Genetic loss of Faah compromises male fertility in mice.

    Xiaofei Sun, Haibin Wang, Masaru Okabe, Kenneth Mackie, Philip J Kingsley, Lawrence J Marnett, Benjamin F Cravatt and Sudhansu K Dey

    Biol Reprod, 80 (2), 235-242, 2009.

Link

http://www.biken.osaka-u.ac.jp/