Department of Molecular Biology(Oriental Yeast Co._Endowed Chair)

Subjects:

1. Constructions of msGW vectors for high-throughput introduction of multiple genes labeled by fluorescence protein tags.
2. Development of proper promoters and IRES signals for expression at physiological levels and development and improvement of fluorescence protein tags for bio imaging.
3. Development of the technology to control the expression levels of introduced genes and quantification methods of the fluorescently tagged products at lower levels.

Published Papers

1. Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system.

   Yukari Sasaki, Takefumi Sone, Shouhei Yoshida, Kazuhide Yahata, Junko Hotta, Jonathan D Chesnut, Takeshi Honda and Fumio Imamoto

   J Biotechnol, 107 (3), 233-243, 2004.

Link

http://www.biken.osaka-u.ac.jp/