Department of Immunoregulation

Subjects:

1. Biochemistry and molecular biology of biosynthesis pathway of GPI-anchored proteins.
2. Molecular pathogenesis of a GPI-anchor deficiency, paroxysmal nocturnal hemoglobinuria.
3. Roles of GPI in protozoan parasites.

Papers published in 2003

1. Requirement of N-glycan on GPI-anchored proteins for efficient binding of aerolysin but not Clostridium septicum alpha-toxin.

   Yeongjin Hong, Kazuhito Ohishi, Norimitsu Inoue, Ji Young Kang, Hiroaki Shime, Yasuhiko Horiguchi, F Gisou van der Goot, Nakaba Sugimoto and Taroh Kinoshita

   EMBO J, 21 (19), 5047-5056, 2002.
2. Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.

   Kazuhito Ohishi, Kisaburo Nagamune, Yusuke Maeda and Taroh Kinoshita

   J Biol Chem, 278 (16), 13959-13967, 2003.
3. Molecular genetics of paroxysmal nocturnal hemoglobinuria.

   Norimitsu Inoue, Yoshiko Murakami and Taroh Kinoshita

   Int J Hematol, 77 (2), 107-112, 2003.
4. Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.

   Yeongjin Hong, Kazuhito Ohishi, Ji Young Kang, Satoshi Tanaka, Norimitsu Inoue, Jun-ichi Nishimura, Yusuke Maeda and Taroh Kinoshita

   Mol Biol Cell, 14 (5), 1780-1789, 2003.
5. [Biosynthesis of GPI-anchored proteins]

   Satoshi Tanaka, Yusuke Maeda and Taroh Kinoshita

   Tanpakushitsu Kakusan Koso, 48 (8 Suppl), 990-996, 2003.
6. GPI transamidase of Trypanosoma brucei has two previously uncharacterized (trypanosomatid transamidase 1 and 2) and three common subunits.

   Kisaburo Nagamune, Kazuhito Ohishi, Hisashi Ashida, Yeonchul Hong, Jun Hino, Kenji Kangawa, Norimitsu Inoue, Yusuke Maeda and Taroh Kinoshita

   Proc Natl Acad Sci U S A, 100 (19), 10682-10687, 2003.
7. PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor.

   Yoshiko Murakami, Uamporn Siripanyapinyo, Yeongjin Hong, Ji Young Kang, Sonoko Ishihara, Hideki Nakakuma, Yusuke Maeda and Taroh Kinoshita

   Mol Biol Cell, 14 (10), 4285-4295, 2003.

Link

http://www.biken.osaka-u.ac.jp/