園田班「動物細胞を使った逆遺伝学によるDNA組み換え、修復、複製の系統的解析」 研究代表者；園田　英一朗 分担者；榎本　武美、大森　治夫 園田　英一朗 Sonoda, E., Okada, T., Zhao, G. Y., Tateishi, S., Araki, K., Yamaizumi, M., Yagi, T., Verkaik, N. S., van Gent, D. C., Takata, M., and Takeda, S. (2003). Multiple roles of Rev3, the catalytic subunit of polzeta in maintaining genome stability in vertebrates. EMBO J. 22, 3188-3197. Morrison, C., Vagnarelli, P., Sonoda, E., Takeda, S., and Earnshaw, W. C. (2003). Sister chromatid cohesion and genome stability in vertebrate cells. Biochem. Soc. Trans. 31, 263-265. Yamashita, Y. M., Okada, T., Matsusaka, T., Sonoda, E., Zhao, G. Y., Araki, K., Tateishi, S., Yamaizumi, M., and Takeda, S. (2002).　RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells. EMBO J. 21, 5558-5566. Tauchi, H., Kobayashi, J., Morishima, K., van Gent, D. C., Shiraishi, T., Verkaik, N. S., vanHeems, D., Ito, E., Nakamura, A., Sonoda, E., et al. (2002). Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells. Nature 420, 93-98. Okada, T., Sonoda, E., Yamashita, Y. M., Koyoshi, S., Tateishi, S., Yamaizumi, M., Takata, M., Ogawa, O., and Takeda, S. (2002). Involvement of vertebrate polkappa in Rad18-independent postreplication repair of UV damage. J. Biol. Chem. 277, 48690-48695. Wang, H., Zeng, Z. C., Bui, T. A., Sonoda, E., Takata, M., Takeda, S., and Iliakis, G. (2001). Efficient rejoining of radiation-induced DNA double-strand breaks in vertebrate cells deficient in genes of the RAD52 epistasis group. Oncogene 20, 2212-2224. Takata, M., Sasaki, M. S., Tachiiri, S., Fukushima, T., Sonoda, E., Schild, D., Thompson, L. H., and Takeda, S. (2001). Chromosome instability and defective recombinational repair in knockout mutants of the five Rad51 paralogs. Mol. Cell. Biol. 21, 2858-2866. Sonoda, E., Morrison, C., Yamashita, Y. M., Takata, M., and Takeda, S. (2001). Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 356, 111-117. Sonoda, E., Takata, M., Yamashita, Y. M., Morrison, C., and Takeda, S. (2001). Homologous DNA recombination in vertebrate cells. Proc. Natl. Acad. Sci. USA 98, 8388-8394. Sonoda, E., Matsusaka, T., Morrison, C., Vagnarelli, P., Hoshi, O., Ushiki, T., Nojima, K., Fukagawa, T., Waizenegger, I. C., Peters, J. M., et al. (2001). Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells. Dev. Cell 1, 759-770. Fukushima, T., Takata, M., Morrison, C., Araki, R., Fujimori, A., Abe, M., Tatsumi, K., Jasin, M., Dhar, P. K., Sonoda, E., et al. (2001). Genetic analysis of the DNA-dependent protein kinase reveals an inhibitory role of Ku in late S-G2 phase DNA double-strand break repair. J. Biol. Chem. 276, 44413-44418. Fujimori, A., Tachiiri, S., Sonoda, E., Thompson, L. H., Dhar, P. K., Hiraoka, M., Takeda, S., Zhang, Y., Reth, M., and Takata, M. (2001). Rad52 partially substitutes for the Rad51 paralog XRCC3 in maintaining chromosomal integrity in vertebrate cells. EMBO J. 20, 5513-5520. Dhar, P. K., Sonoda, E., Fujimori, A., Yamashita, Y. M., and Takeda, S. (2001). DNA repair studies: experimental evidence in support of chicken DT40 cell line as a unique model. J. Environ. Pathol. Toxicol. Oncol. 20, 273-283. 大森　治夫 Ishikawa T., Uematsu N., Mizukoshi T., Iwai S., Iwasaki H., Masutani C., Hanaoka F., Ueda R., Ohmori H. & Todo T. (2001) Mutagenic and non-mutagenic bypass of DNA lesions by Drosophila DNA polymerases dpol h and dpol i. J. Biol. Chem. 276, 15155-15163 Levine RL., Miller H., Grollman A., Ohashi E., Ohmori H., Masutani C., Hanaoka F. & Moriya M. (2001)　Translesion DNA synthesis catalyzed by human polh and polk across 1,N6-ethenodeoxyadenosine. J. Biol. Chem. 276, 18717-18721 Ohmori H., Friedberg EC., Fuchs RPP., Goodman MF., Hanaoka F., Hinkle D., Kunkel TA., Lawrence CW., Livneh Z., Nohmi T., Prakash L., Prakash S., Todo T., Walker GC., Wang Z. &, Woodgate R. (2001)　　The Y-family of DNA polymerases. Mol. Cell 8, 7-8. O-Wang J., Kawamura K., Tada Y., Ohmori H., Kimura H, Sakiyama S. & Tamura, M. (2001) DNA polymerase k, implicated in spontaneous and DNA damage-induced mutagenesis, is overexpressed in lung cancer. Cancer Res. 61, 5366-5369. Ogi T., Mimura J., Hikida M., Fujimoto H., Fujii-Kuriyama Y. & Ohmori H. (2001) Expression of human and mouse genes encoding polk: testis-specific developmental regulation and AhR-dependent inducible transcription. Genes Cells 6, 943-953. Burgers PMJ., Koonin EV., Bruford E., Blanco L., Burtis KC., Christman MF., Copeland WC., Friedberg EC., Hanaoka F., Hinkle DC, Lawrence CW, Nakanishi M, Ohmori H., Prakash L., Prakash S., Reynaud C-A., Sugino A., Todo T, Wang Z. & Woodgate R. (2001) Eukaryotic DNA polymerases: proposed for a revised nomenclature. J. Biol. Chem. 276, 43487-43490 Suzuki N., Ohashi E., Hayashi K., Ohmori H, Grollman AP. & Shibutani S. (2001) Translesion synthesis past acetylaminofluorene-derived DNA adducts catalyzed by human DNA polymerase k and Escherichia coli DNA polymerase IV. Biochemistry 40, 15176-15183 Suzuki N., Ohashi E., Kolbanovskiy A,, Geacintov NE., Grollman AP., Ohmori H. & Shibutani S. (2002) Translesion synthesis by human DNA polymerase kk on a DNA template containing a single stereoisomer of dG-(+)- or dG-(-)-anti-N(2)-BPDE (7,8-dihydroxy-anti-9,10-epoxy- 7,8,9,10-tetrahydrobenzo[a]pyrene). Biochemistry 41, 6100-6106 Ogi T., Shinkai Y., Tanaka K. & Ohmori H. (2002) Polk protects mammalian cells against the lethal and mutagenic effects of benzo[a]pyrene. Proc Natl Acad Sci U S A. 99, 15548-15553 Frank, EG., Sayer JM., Ohashi E., Ohmori H., Jerina DM. & Woodgate R. (2002) Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxadenosine and deoxyguanosine by human DNA polymerase i. Nucleic Acids Res. 30, 5284-5292 Yang IY., Miller H., Wang Z., Frank EG., Ohmori H., Hanaoka F. & Moriya M (2003) Mammalian translesion DNA synthesis across an acrolein-derived deoxyguanosine adduct. Participation of DNA polymerase h in error-prone synthesis in human cells. J Biol Chem. 278, 13989-13994 Shimizu T., Shinkai Y., Ogi T., Ohmori H. & Azuma T. (2003) The absence of DNA polymerase k does not affect somatic hypermutation of the mouse immunoglobulin heavy chain gene. Immunol Lett. 86, 265-270 Vaisman A., Frank, EG., Iwai S., Ohashi E., Ohmori H., Hanaoka F. & Woodgate R. (2003) Sequence context-dependent replication of DNA templates containing UV-induced lesion by human DNA polymerase i. DNA Repair in press 榎本　武美 Onoda, F., Seki, M., Miyajima, A., and Enomoto, T. (2001) Involvement of SGS1 in DNA damage-induced heteroallelic recombination that requires RAD52. Mol. Gen. Gnet. 264, 702-708 Enomoto, T. (2001) Functions of RecQ family helicases: Possible involvement of Bloom's and Werner's syndrome gene products in guarding genome integrity during DNA replication. J. Biochem. 129, 501-507 Kawabe, Y., Branzei, D., Suzuki, H., Masuko, T., Onoda, F., Heo, S.-J., Ikeda, H., Shimamoto, A., Fruichi, Y., Seki, M., and Enomoto, T. (2001) A novel protein interacts with the Werner's syndrome gene product physically and functionally. J. Biol. Chem. 276, 20364-20369 Ui, A., Satoh, Y., Onoda, F., Miyajima, A., Seki, M., and Enomoto, T. (2001) The N-terminal region of Sgs1, which interacts with Top3, is required for complementation of MMS sensitivity and suppression of hyper-recombination in sgs1 disruptants. Mol. Genet. Genomics 265, 837-850 Hachiya, Y., Motonaga, K., Itoh, M., Masuko, T., Enomoto, T., Sonobe, H., and Takashima, S. (2001) Immunohistochemical expression and pathogenesis of BLM in human brain and visceral organs. Neuropathol. 21, 1123-128 Tada, S., Kobayashi, T., Omori, A., Kusa, Y., Okumura, N., Kodaira, H., Ishimi, Y., Seki, M., and Enomoto, T. (2001) Molecular cloning of a cDNA encoding mouse DNA helicase B, which has homology to Escherichia coli RecD protein, and identification of a mutation in the DNA helicase B from tsFT848 temperature-sensitive DNA replication mutant cells. Nuc. Acids Res. 29, 3835-3840 Suzuki, H., Seki, M., Kobayashi, T., Kawabe, Y., Kaneko, H., Kondo, N., Harata, M., Mizuno, S., Masuko, T., and Enomoto, T. (2001) The N-terminal internal region of BLM is required for the formation of dots/rod-like structures which are associated with SUMO-1. Biochem. Biophys. Res. Commun. 286, 322-327 Onodera, R., Seki, M., Ui, A., Satoh, Y., Miyajima, A., Onoda, F., and Enomoto, T. (2002) Functional and physical interaction between Sgs1 and Top3 and Sgs1-independent function of Top3 in DNA recombination repair. Genes Genet. Syst. 77, 11-21 Kobayashi, T., Tada, S., Tsuyama, T., Murofushi, H., Seki, M., and Enomoto, T. (2002) Effect of topoisomerase inhibitors on the formation of replication protein A-foci in nuclei reconstituted in Xenopus egg extract. J. Health Sci. 48, 296-301 Kobayashi, T., Tada, S., Tsuyama, T., Murofushi, H., Seki, M., and Enomoto, T. (2002) Focus-formation of replication protein A, activation of checkpoint system and DNA repair synthesis induced by DNA double-strand breaks in Xenopus egg extract. J. Cell Sci. 115, 3159-3169 Branzei, D., Seki, M., Onoda, F., Yagi, H., Kawabe, Y.-i., and Enomoto, T. (2002) Characterization of the slow-growth phenotype of S. cerevisiae whip/mgs1 sgs1 double deletion mutants. DNA Repair 1, 671-682 Hayashi, T., Seki, M., Maeda, D., Wang, W., Kawabe, Y.-i., Seki, T., Fukagawa, T., Yagi, H., and Enomoto, T. (2002) Ubc9 is essential for viability of higher eukaryotic cells. Exp. Cell Res. 280, 212-221. Branzei, D., Seki, M., Onoda, F., and Enomoto, T. (2002) Saccharomyces cerevisiae WHIP/MGS1 gene interacts genetically with DNA replication genes. Mol. Genet. Genomics 268, 371-386 Odagiri, N., Seki, M., Onoda, F., Yoshimura, A., Watanabe, S., and Enomoto, T. (2003) Budding yeast mms4 is epistatic with rad52 and its function can be replaced by a bacterial Holliday junction resolvase. DNA Repair 2, 347-358. Wang, W., Seki, M., Narita, Y., Nakagawa, T., Yoshimura, A., Otsuki, M., Kawabe, Y-i., Tada, S., Yagi, H., Ishii, Y., and Enomoto, T. (2003) Functional relation among RecQ family helicases, RecQL1, RecQL5, and BLM in cell growth and SCE formation. Mol. Cell. Biol. 23, 3527-3535 Isik, S., Sano, K., Tsutsui, K., Seki, M., Enomoto, T., Saitoh, H., Tsutsui, K. (2003) The SUMO pathway is required for selective degradation of DNA topoisomerase IIb induced by a catalytic inhibitor ICRF-193. FEBS Lett. 546, 374-378 back