研究業績

	品川班「組み換えと複製におけるHolliday構造の形成と解離の機構」研究代表者；品川　日出夫分担者；岩崎　博史、石野　良純、森川　耿右、菱田　卓、森下　卓 Hishida T, Iwasaki H, Ohno T, Morishita T, Shinagawa H. (2001) A yeast gene, MGS1, encoding a DNA-dependent AAA(+) ATPase is required to maintain genome stability. Proc. Natl. Acad. Sci. USA. 98 :8283-8289. Yamada K., Kunishima N., Mayanagi K., Ohnishi T., Nishino T., Iwasaki H., Shinagawa H., and Morikawa K. (2001)Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8. Proc. Natl. Acad. Sci. USA 98/1442-1447 Yoshikawa M., Iwasaki H., Shinagawa H. (2001) Evidence that Phenylalanine 69 in Escherichia coli RuvC Resolvase forms a Stacking　Interaction during Binding and Destabilization of a Holliday Junction DNA Substrate.　 J. Biol. Chem. 276: 10432-10436. Han Y-W, Iwasaki H, Miyata T, Mayanagi K, Yamada K, Morikawa K, Shinagawa H. (2001)　A unique beta-hairpin protruding from AAA+ ATPase domain of RuvB motor protein is involved in the interaction with RuvA DNA recognition protein for branch migration of Holliday junctions. J. Biol. Chem. 276 : 35024-35028. Oyama T., Ishino Y., Cann I., Ishino S., Morikawa K. (2001) Atomic structure of the clamp loader small subunit from Pyrococcus furiosus. Mol. Cell 8 : 455-463. Matsunaga F., Forterre P., Ishino Y., Myllykallio H. (2001) In vivo interactions of archaeal Cdc6/Orcl and minichromosome maintenance proteins with the replication origin. Proc. Natl. Acad. Sci. USA 98 : 11152-11157. Liu L., Komori K., Ishino S., Bocquier A., Cann I., Kohda D., Ishino Y. (2001) The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus. 　 J. Biol. Chem. 276 : 45484-45490. Tsutsui Y, Khasanov FK, Shinagawa H, Iwasaki H, Bashkirov VI. (2001) Multiple interactions among the components of the recombinational DNA repair system in Schizosaccharomyces pombe.　 Genetics 159 : 91-105. Hishida T, Ohno T, Iwasaki H, Shinagawa H. (2002) Saccharomyces cerevisiae MGS1 is essential in strains deficient in the RAD6-dependent DNA damage tolerance pathway.　 EMBO J. 21 : 2019-2029. Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K (2002) Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery.　 Mol. Cell. 10 : 671-681. Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H. (2002) The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents.　 Mol. Cell. Biol. 22 : 3537-3548. Takamatsu C, Umeda S, Ohsato T, Ohno T, Abe Y, Fukuoh A, Shinagawa H, Hamasaki N, Kang D. (2002) Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein.　 EMBO Rep. 3 :451-456. Komori K, Fujikane R, Shinagawa H, Ishino Y. (2002) Novel endonuclease in Archaea cleaving DNA with various branched structure. Genes Genet. Syst. 77 : 227-241. Iida T, Makino K, Nasu H, Yokoyama K, Tagomori K, Hattori A, Okuno T, Shinagawa H, Honda T. (2002) Filamentous bacteriophages of vibrios are integrated into the dif-like site of the host chromosome.　 J. Bacteriol. 184 : 4933-4935. Daimon K., Kawarabayasi Y., Kikuchi H., Sako Y., Ishino Y. (2002) Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon, Aeropyrum pernix: interactions with the two DNA polymerases. J. Bacteriol. 184 : 687-694. Matsumiya S., Ishino S., Ishino Y., Morikawa K. (2002) Physical interaction between proliferating cell nuclear antigen and replication factor C from Pyrococcus furiosus. Genes Cells 7 : 911-922. Sha R, Liu F, Iwasaki H, Seeman NC. (2002) Parallel symmetric immobile DNA junctions as substrates for E. coli RuvC Holliday junction resolvase.　 Biochemistry 41 : 10985-10993. Prabhu VP, Simons AM, Iwasaki H, Gai D, Simmons DT, Chen J. (2002) p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC. J. Mol. Biol. 316 : 1023-1032. Tomita K, Matsuura A, Caspari T, Carr AM, Akamatsu Y, Iwasaki H, Mizuno K, Ohta K, Uritani M, Ushimaru T, Yoshinaga K, Ueno M. (2003) Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres. Mol. Cell. Biol. 23 :5186-5197. Nishino, T., Komori, K., Ishino, Y., & Morikawa, K. (2003) X-ray structural and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: a novel domain arrangement and a striking similarity between its central endonuclease domain and type II restriction enzymes. Structure 11 : 445-457. Ishino, S., Oyama, T., Yuasa, M., Morikawa, K. & Ishino, Y. (2003) Mutational Analysis of Pyrococcus furiosus Replication Factor C based on the Three-Dimensional Structure. Extremophiles 7 : 169-175. Matsumiya, S., Ishino, S., Ishino, Y., & Morikawa, K. (2003) Intermolecular ion pairs maintain toroidal structure of Pyrococcus furiosus PCNA. Prot. Natl. Acad. Sci. 12 : 823-831. Jolivet, E., Matsunaga, F., Ishino, Y., Forterre, P., Prieur, D., & Myllykallio, H. (2003) Physiological responses of the hyperthermophilic archaeon Pyrococus abyssi to DNA damage caused by ionizing radiation. J. Bacteiol. 185 : 3958-3961. Hishida T., Iwasaki H., Han H-W, Ohnishi T., Shinagawa H. (2003) Uncoupling of the ATPase activity from the branch migration activity of RuvAB protein complexes containing both wild-type and ATPase-defective RuvB proteins　　 Genes to Cells (in press). Ueno M., Nakazaki T., Akamatsu Y., Watanabe K., Tomita K., Lindsay H.D., Shinagawa H., Iwasaki H. (2003) Molecular characterization of the Schizosaccharomyces pombe nbs1+ gene involved in DNA repair and telomere maintenance. Mol. Cell. Biol. (in press). 総説岩崎博史、品川日出夫 (2001)　ホリディ構造とDNA組換え修復蛋白質核酸酵素 46 : 995-1003. 森下　卓、品川日出夫 (2002)　DNA二重鎖切断と組換え修復実験医学 20 : 45(1539)-49(1543). 品川日出夫 (2003)　相同的DNA組換え中間体ホリディ構造の解離の機構蛋白質核酸酵素 48 : 727-732. 特許品川　日出夫、石野　良純　　　　　　　　件名：特開　2002-288726 　　　　　　　条件：新規DNAヘリカーゼ石野　良純　　件名：特開　2001-337530 　　　　　　　条件：新規DNAポリメラーゼ補助因子　　　　　　　件名：特開　2001-372434 　　　　　　　条件：新規DNA分解酵素 back