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新着情報
  • Toshitsugu Fujita received the Young Investigator Award of the Japanese Society for Epigenetics. (April 22, 2016) The 10th Annual Meeting of the Japanese Society for Epigenetics
  • We published the paper on in vitro enChIP. (February 5, 2016) Genes Cells
  • The enChIP kit has been released from Active Motif, Inc. (February 3, 2016) Active Motif
  • We published a video article showing step-by-step instructions of the enChIP analysis. (January 20, 2016) JoVE
  • We published a review on the locus-specific ChIP technology. (January 18, 2016) Gene Regul Syst Bio
  • We developed the enChIP-Seq technology to detect physical interactions between genomic regions. (January 7, 2016) bioRxiv
  • We developed the in vitro enChIP technology using the recombinant CRISPR ribonucleoproteins. (November 30, 2015) bioRxiv
  • We published a review on applications of engineered DNA-binding molecules such as TAL proteins and the CRISPR/Cas system in biological research. (September 24, 2015) Int. J. Mol. Sci.
  • We published a review on the enChIP technology. (September 9, 2015) Int. J. Mol. Sci.
  • On April 16, 2015, we founded a bio-venture, "Epigeneron, LLC", to develop epigenetic drugs using locus-specific ChIP technologies. (July 15, 2015)
  • Hodaka Fujii received the Osaka University Presidential Award for Encouragement. (July 14, 2015) Osaka University
  • Hodaka Fujii gave a webinar on enChIP. (June 8, 2015) EpiGenie
  • By combining enChIP with RNA sequencing (enChIP-RNA-Seq), we identified RNAs associated telomeres. To our knowledge, this study is the first non-biased identification of RNAs associated with specific genomic regions. (April 13, 2015) PLoS One
  • We published a review on analysis of molecular mechanisms of genome functions using enChIP. (April 12, 2015) PubMed
  • An expression plasmid of the FLAG-tagged dCas9 for the enChIP analysis in budding yeast is now available through Addgene.(February 25, 2015) Addgene
  • We identified proteins associated with the Pax5 promoter by using iChIP and elucidated mechanisms of B cell-specific expression of Pax5. (January 21, 2015) PLoS One
  • An interview article of Hodaka Fujii has been posted at Addgene Blog. (December 3, 2014) Addgene Blog
  • We published a paper on the in vitro iChIP using the recombinant LexA protein. (December 3, 2014) BMC Mol. Biol.
  • We have developed the ORNi-PCR method to specifically inhibit PCR amplification by using oligoribonucleotides (ORNs). (November 18, 2014) PLoS One
  • We identified proteins associated with an IFNγ-inducible promoter by using the enChIP-SILAC technology. (July 22, 2014) PLoS One
  • Hodaka Fujii joined the Editorial Board of PLoS One. (July 21, 2014) PLoS One
  • Hodaka Fujii received the Osaka University Presidential Award for Encouragement. (July 8, 2014) Osaka University
  • Hodaka Fujii joined the Editorial Board of Scientific Reports. (June 24, 2014) Sci. Rep.
  • Hodaka Fujii joined the Advisory Board of Addgene. (June 16, 2014) Addgene
  • We developed the in vitro iChIP technology using the recombinant LexA protein. (June 7, 2014) bioRxiv
  • We published a detailed protocol of enChIP using TAL proteins. (May 20, 2014) bio-protocol
  • The Japan patent on iChIP has been registered (Japan patent No. 5413924 "Method for isolating specific genomic regions"). (Nov. 22, 2013))
  • We have developed enChIP using TAL proteins and purified mammalian telomeres to identify their interacting molecules. (Nov. 8, 2013) Sci. Rep.
  • We have updated our website. (Oct. 23, 2013)
  • The paper reporting development of enChIP is now the most downloaded article in Biochem. Biophys. Res. Commun.. (Oct. 8, 2013) Biochem. Biophys. Res. Commun.
  • We start distribution of our plasmids including those for enChIP analysis through Addgene. (Sep. 14, 2013)
    Link to Hodaka Fujii Lab PlasmidsFind Hodaka Fujii Lab Plasmids
  • We have developed engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) to purify specific genomic regions. enChIP does not require insertion of recognition sequences of exogenous DNA-bindng molecules, making the purification procedure much more straightforward. (Aug. 11, 2013) Biochem. Biophys. Res. Commun.
  • The US patent on iChIP has been registered (US 8,415,098 B2 "Method for isolating specific genomic regions"). (Apr. 9, 2013))US8415098
  • We have published a review on iChIP. (Jan. 15, 2013) ISRN Biochem.
  • We have updated our website. (Nov. 24, 2012)
  • We have developed a second-generation tagged LexA DNA-binding protein for the iChIP analysis. This newer system markedly increases the yield of purification of specific genomic regions. (Oct. 17, 2012) Adv. Biosci. Biotechnol.
  • We have determined transcription start sites of mouse Foxp3 gene and analyzed the usage of its first exons. (Jun. 27, 2012) PubMed
  • We have succeeded in directly identifying components of insulator complexes, which function as boundaries of genomic regions, by using iChIP. This is the first example of direct identification of proteins and RNA associated with specific genomic regions of interest in vivo. (Oct. 18, 2011) PLoS ONE
  • We have characterized 5'-genomic structure and determined transcription start sites of chicken Pax5 gene. PubMed
  • We have developed insertional chromatin immunoprecipitation (iChIP) to purify specific genomic regions. This novel technology will be a useful tool for elucidation of epigenetic regulation. PubMed