Maeda Lab/International Research Center for Infectious Diseases  Laboratory of Viral Dynamism Research

The virus dynamism research group studies RNA viruses such as chikungunya virus (CHIKV) and severe fever with thrombocytopenia syndrome virus (SFTSV) as model viruses, and conducts research aimed at understanding and controlling the mechanisms by which infectious diseases develop from the interaction between the virus by identification of host factors involved in entry, proliferation, and release of these viruses.

 

In order to accomplish the above purposes, our original assay systems such as trans-replication and reporter cells have been constructed and combined with molecular biological techniques such as genome-wide screening (reverse genetics) and gene knockout/knockdown (forward genetics), cell biological techniques such as microscopic analysis of organella and vesicle transport, and biochemical techniques such as protein-protein interaction and post-protein translational modification (such as sugar chain and ADP-ribosylation) analysis. Thus, to analyze virus-host interactions, we are combining our own assay system with a variety of techniques in various fields. We have also started research on the control of a new coronavirus, which is an urgent social issue, as part of the contribution of RIMD to society.

Staff

  • SA Prof.: Yusuke Maeda
  • Postdoc: Yusuke Yamamoto
  • Postdoc: YAN LU

Publications

  • (1) Hepatitis C virus modulates signal peptide peptidase to alter host protein processing. Hirano J. et al. 2021 Proc. Natl. Acad. Sci. U S A 118 (22) e2026184118.

    (2) Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction. Torii S. et al. 2021Cell Rep. 35(3):109014.

    (3) Anti-chikungunya virus monoclonal antibody inhibiting viral fusion and release. Tumkosit U. et al. 2020 J Virol. 94(19):e00252-20.

    (4) Genome-wide screening uncovers the significance of N-sulfation of heparan sulfate as a host cell factor for Chikungunya virus infection. Tanaka A. et al. 2017 J. Virol. 91(13):00432-17.

    (5) GPI-glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi. Fujita M. et.al 2009 Cell 139(2): 352-365

    (6) GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Maeda Y. et al. 2008 Nat. Cell Biol. 10(10): 1135-45.