Horiguchi Lab／Division of Infectious Disease Department of Molecular Bacteriology
Some pathogenic bacteria cause specific disease symptoms including flaccid/spastic paralyses, paroxysmal coughing, skin exfoliation, and osteogenesis imperfecta, besides general symptoms such as fever and inflammation. Our major questions are as to how these specific symptoms appear in response to bacterial infections or what kinds of bacterial virulence factors are involved in them. We hope that we will understand the nature of bacterial infections by answering these questions.
To understand the mechanism of infection
Bordetella pertussis, which is one of our research subjects is a representative pathogenic bacteria of Bordetella and causes whooping cough. In addition to B. pertussis, B. parapertussis and B. bronchiseptica are categorized in the genus Bordetella. Although these pathogenic organisms share homologous virulence factors and commonly cause respiratory infections with characteristic coughing, their host specificities and the course of disease manifestation are quite different: B. pertussis is a strict human pathogen causing the acute disease whereas B. bronchiseptica infects a wide range of mammals and causes chronic infections. We are trying to understand what determines host specificities and distinct disease manifestations in Bordetella infections. Understanding the molecular mechanism by which the bacteria cause coughing in hosts is our another goal of the Bordetella research.
Analyzing the structure-function relationship of bacterial protein toxins.
Bacterial protein toxins cause a variety of specific symptoms manifested in bacterial infections. Many bacterial protein toxins are essentially multifunctional biomolecules, which travel in a host body, bind to target molecules or cells, and modify target molecules with high specificity. Some bacterial toxins are known as the most poisonous substances on the earth. We are analyzing the structure-function relationship of these bacterial protein toxins to understand how they exert such powerful toxicities on target cells and intoxicated animals. We believe that these results should give an insight into the mechanism causing specific symptoms observed in bacterial infections.
To achieve the above-mentioned goals, we are conducting the research work by using every experimental technique based on bacteriology, molecular and cellular biology, biochemistry, medical and veterinary science.
- Prof.: Yasuhiko Horiguchi
- Asst. Prof.: Yukihiro Hiramatsu
- Asst. Prof.: Takashi Nishida
- Postdoc: Dendi Krisna Nugraha
- (1)Interference of flagellar rotation up-regulates the expression of small RNA contributing to Bordetella pertussis infection. Hiramatsu Y. et al., Science Advances (2022) 8(51):eade8971
(2)The Mechanism of Pertussis Cough Revealed by the Mouse-Coughing Model. Hiramatsu Y. et al., mBio (2022) 13(2):e0319721
(3)Bordetella dermonecrotic toxin is a neurotropic virulence factor that uses CaV3.1 as the cell surface receptor. Teruya S. et al. mBio (2020)11:e03146-19.
(4)Bordet‐Gengou agar medium supplemented with albumin‐containing biologics for cultivation of bordetellae. Hiramatsu Y. et al. Microbiology and Immunology (2019) 63(12):513-516.
(5)BspR/BtrA, an anti-σ factor, regulates the ability of Bordetella bronchiseptica to cause cough in rats. Nakamura K. et al. mSphere (2019) 4:e00093-19
(6)The Eukaryotic Host Factor 14-3-3 Inactivates Adenylate Cyclase Toxins of Bordetella bronchiseptica and B. parapertussis, but not B. pertussis. Fukui-Miyazaki A., et al., mBio (2018) 9:49–15.
(7) Ectopic Expression of O Antigen in Bordetella pertussis by a Novel Genomic Integration System. Ishigaki K., et al., mSphere (2018) 3:e00417–17–11.
(8) The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica. Nishikawa S., et al., Microbiology and Immunology (2016)60(2)93-105.