Department of Experimental Genome Research

Staff:

Professor

Masahito Ikawa, Ph.D.

Specially Appointed Associate Professor(SUP)

Ayako Isotani, Ph.D.

Assistant Professor

Yuhkoh Satouh, Ph.D.

Yoshitaka Fujihara, Ph.D.

Haruhiko Miyata, Ph.D.

Masashi Mori, Ph.D.

Specially Appointed Assistant Professor(SUP)

Daiji Kiyozumi, Ph.D.

JSPS Postdoctoral Fellow

Taichi Noda, Ph.D.

Specially Appointed Researcher

Qian Zhang, Ph.D.

Keisuke Shimada, D.V.M., Ph.D.

Kanako Kita, Ph.D.

Julio Manuel Castaneda, Ph.D.

Educational Support Staff

Kazuhiro Kaseda

Technical Staff

Ryuta Inoue

Specially Appointed Technical Staff(SUP)

Mitsuru Yamane

Saori Haga

Yoko Horiguchi

Mutsumi Yamane

Yuko Oki, D.V.M

Graduate Student

Kaori Nozawa, D.V.M.

Masanaga Muto

Asami Oji

Tomohiro Tobita

Takafumi Matsumura

Ferheen Abbasi

Undergraduate Student

Seiya Oura

Kiyonori Kobayashi

Tamara Larasati

Subjects:

1. Analysis of molecular mechanisms involved in mammalian gametogenesis, fertilization, and implantation.
2. Development of new technologies to produce genetically manipulated animals. (CRISPR/Cas system, lentiviral vectors, etc.).
3. Analysis of interspecies cell-cell communication in xenogenic chimeric animals.

Papers published in 2016

1. STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation.

   Ken Takashima, Yohei Takeda, Hiroyuki Oshiumi, Hiroaki Shime, Masaru Okabe, Masahito Ikawa, Misako Matsumoto and Tsukasa Seya

   Biochem Biophys Res Commun, 478 (4), 1764-1771, 2016.
2. CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility.

   Samantha A M Young, Haruhiko Miyata, Yuhkoh Satouh, Masanaga Muto, Martin R Larsen, R John Aitken, Mark A Baker and Masahito Ikawa

   Reproduction, 152 (6), 665-672, 2016.
3. CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

   Samantha A M Young, Haruhiko Miyata, Yuhkoh Satouh, Robert John Aitken, Mark A Baker and Masahito Ikawa

   J Cell Sci, 129 (23), 4379-4387, 2016.
4. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.

   Gun-Hee Lee, Morihisa Fujita, Katsuyoshi Takaoka, Yoshiko Murakami, Yoshitaka Fujihara, Noriyuki Kanzawa, Kei-Ichi Murakami, Eriko Kajikawa, Yoko Takada, Kazunobu Saito, Masahito Ikawa, Hiroshi Hamada, Yusuke Maeda and Taroh Kinoshita

   J Cell Biol, 215 (5), 705-718, 2016.
5. Human HLA-Ev (147) Expression in Transgenic Animals.

   R Matsuura, A Maeda, R Sakai, H Eguchi, P-C Lo, H Hasuwa, M Ikawa, K Nakahata, M Zenitani, T Yamamichi, S Umeda, K Deguchi, H Okuyama and S Miyagawa

   Transplant Proc, 48 (4), 1323-1325, 2016.
6. Knockout of Cytidine Monophospho-N-Acetylneuraminic Acid (CMP-NeuAc) Hydroxylase From Porcine Endothelial Cells by a CRISPR System.

   R Sakai, Y Esaki, H Hasuwa, M Ikawa, P Lo, R Matsuura, K Nakahata, M Zenitani, M Asada, A Maeda, H Eguchi, H Okuyama and S Miyagawa

   Transplant Proc, 48 (4), 1320-1322, 2016.
7. Expression of a Synthetic Gene of CTDM by Transgenic Animals.

   R Sakai, A Maeda, R Matsuura, H Eguchi, P Lo, H Hasuwa, M Ikawa, K Nakahata, M Zenitani, T Yamamichi, S Umeda, H Okuyama and S Miyagawa

   Transplant Proc, 48 (4), 1279-1281, 2016.
8. Impaired female fertility in tubulointerstitial antigen-like 1-deficient mice.

   Akihito Takahashi, Ajalli Rahim, Miki Takeuchi, Emiko Fukui, Midori Yoshizawa, Kuniaki Mukai, Makoto Suematsu, Hidetoshi Hasuwa, Masaru Okabe and Hiromichi Matsumoto

   J Reprod Dev, 62 (1), 43-49, 2016.
9. The Acrosome Reaction: A Historical Perspective.

   Masaru Okabe

   Adv Anat Embryol Cell Biol, 220, 1-13, 2016.
10. Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318-knockout mice.

    Masamichi Ishizuka, Eri Ohtsuka, Atsuto Inoue, Mirei Odaka, Hirotaka Ohshima, Norihisa Tamura, Kaoru Yoshida, Norihisa Sako, Tadashi Baba, Shin-Ichi Kashiwabara, Masaru Okabe, Junko Noguchi and Hiromi Hagiwara

    Dev Growth Differ, 58 (7), 600-608, 2016.
11. The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization after Natural Mating.

    Toshiaki Hino, Yuko Muro, Miwa Tamura-Nakano, Masaru Okabe, Hiroyuki Tateno and Ryuzo Yanagimachi

    Biol Reprod, 95 (3), 50, 2016.
12. Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naïve from primed pluripotent stem cells.

    Shin Kobayashi, Yusuke Hosoi, Hirosuke Shiura, Kazuo Yamagata, Saori Takahashi, Yoshitaka Fujihara, Takashi Kohda, Masaru Okabe and Fumitoshi Ishino

    Development, 143 (16), 2958-2964, 2016.
13. Immunomodulatory drugs inhibit TLR4-induced type-1 interferon production independently of Cereblon via suppression of the TRIF/IRF3 pathway.

    David Millrine, Haruhiko Miyata, Mami Tei, Praveen Dubey, Kishan Nyati, Taisuke Nakahama, Yohannes Gemechu, Barry Ripley and Tadamitsu Kishimoto

    Int Immunol, 28 (6), 307-315, 2016.
14. The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.

    Daisuke Yamazaki, Haruhiko Miyata, Yosuke Funato, Yoshitaka Fujihara, Masahito Ikawa and Hiroaki Miki

    J Cell Sci, 129 (9), 1940-1949, 2016.
15. Generation of Hprt-disrupted rat through mouse←rat ES chimeras.

    Ayako Isotani, Kazuo Yamagata, Masaru Okabe and Masahito Ikawa

    Sci Rep, 6, 24215, 2016.
16. Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation.

    Daisuke Yamazaki, Yosuke Funato, Haruhiko Miyata, Masahito Ikawa and Hiroaki Miki

    Biochem Biophys Res Commun, 474 (3), 441-446, 2016.
17. Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.

    Yoshinori Kanemori, Yoshitaka Koga, Mai Sudo, Woojin Kang, Shin-Ichi Kashiwabara, Masahito Ikawa, Hidetoshi Hasuwa, Kiyoshi Nagashima, Yu Ishikawa, Narumi Ogonuki, Atsuo Ogura and Tadashi Baba

    Proc Natl Acad Sci U S A, 113 (26), E3696-3705, 2016.
18. Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.

    Haruhiko Miyata, Julio M Castaneda, Yoshitaka Fujihara, Zhifeng Yu, Denise R Archambeault, Ayako Isotani, Daiji Kiyozumi, Maya L Kriseman, Daisuke Mashiko, Takafumi Matsumura, Ryan M Matzuk, Masashi Mori, Taichi Noda, Asami Oji, Masaru Okabe, Renata Prunskaite-Hyyrylainen, Ramiro Ramirez-Solis, Yuhkoh Satouh, Qian Zhang, Masahito Ikawa and Martin M Matzuk

    Proc Natl Acad Sci U S A, 113 (28), 7704-7710, 2016.
19. Regulation of intestinal homeostasis by the ulcerative colitis-associated gene RNF186.

    Kosuke Fujimoto, Makoto Kinoshita, Hiroo Tanaka, Daisuke Okuzaki, Yosuke Shimada, Hisako Kayama, Ryu Okumura, Yoki Furuta, Masashi Narazaki, Atsushi Tamura, Shigetsugu Hatakeyama, Masahito Ikawa, Kiichiro Tsuchiya, Mamoru Watanabe, Atsushi Kumanogoh, Sachiko Tsukita and Kiyoshi Takeda

    Mucosal Immunol, 10 (2), 446-459, 2016.
20. Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis.

    Midori Shimada, Takahiro Goshima, Hiromi Matsuo, Yoshikazu Johmura, Mayumi Haruta, Kazuhiro Murata, Hiromitsu Tanaka, Masahito Ikawa, Keiko Nakanishi and Makoto Nakanishi

    Nat Commun, 7, 12059, 2016.
21. Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.

    Kazuki Kato, Yuhkoh Satouh, Hiroshi Nishimasu, Arisa Kurabayashi, Junko Morita, Yoshitaka Fujihara, Asami Oji, Ryuichiro Ishitani, Masahito Ikawa and Osamu Nureki

    Nat Commun, 7, 12198, 2016.
22. CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice.

    Asami Oji, Taichi Noda, Yoshitaka Fujihara, Haruhiko Miyata, Yeon Joo Kim, Masanaga Muto, Kaori Nozawa, Takafumi Matsumura, Ayako Isotani and Masahito Ikawa

    Sci Rep, 6, 31666, 2016.
23. GPI-AP release in cellular, developmental, and reproductive biology.

    Yoshitaka Fujihara and Masahito Ikawa

    J Lipid Res, 57 (4), 538-545, 2016.
24. Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.

    N G Brukman, H Miyata, P Torres, D Lombardo, J J Caramelo, M Ikawa, V G Da Ros and P S Cuasnicú

    Mol Hum Reprod, 22 (4), 240-251, 2016.
25. Ground-based assessment of JAXA mouse habitat cage unit by mouse phenotypic studies.

    Miki Shimbo, Takashi Kudo, Michito Hamada, Hyojung Jeon, Yuki Imamura, Keigo Asano, Risa Okada, Yuki Tsunakawa, Seiya Mizuno, Ken-Ichi Yagami, Chihiro Ishikawa, Haiyan Li, Takashi Shiga, Junji Ishida, Juri Hamada, Kazuya Murata, Tomohiro Ishimaru, Misuzu Hashimoto, Akiyoshi Fukamizu, Mutsumi Yamane, Masahito Ikawa, Hironobu Morita, Masahiro Shinohara, Hiroshi Asahara, Taishin Akiyama, Nobuko Akiyama, Hiroki Sasanuma, Nobuaki Yoshida, Rui Zhou, Ying-Ying Wang, Taito Ito, Yuko Kokubu, Taka-Aki K Noguchi, Hisako Ishimine, Akira Kurisaki, Dai Shiba, Hiroyasu Mizuno, Masaki Shirakawa, Naoki Ito, Shin Takeda and Satoru Takahashi

    Exp Anim, 65 (2), 175-187, 2016.
26. Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway.

    Tomoki Kai, Yoshiyuki Tsukamoto, Naoki Hijiya, Akinori Tokunaga, Chisato Nakada, Tomohisa Uchida, Tsutomu Daa, Hidekatsu Iha, Mika Takahashi, Takeo Nomura, Fuminori Sato, Hiromitsu Mimata, Masahito Ikawa, Masao Seto, Keiko Matsuura and Masatsugu Moriyama

    J Pathol, 239 (1), 97-108, 2016.
27. Calreticulin Regulates Neointima Formation and Collagen Deposition following Carotid Artery Ligation.

    Kurt A Zimmerman, Dongqi Xing, Manuel A Pallero, Ailing Lu, Masahito Ikawa, Leland Black, Kenneth L Hoyt, Janusz H Kabarowski, Marek Michalak and Joanne E Murphy-Ullrich

    J Vasc Res, 52 (5), 306-320, 2015.
28. Deficiency in WT1-targeting microRNA-125a leads to myeloid malignancies and urogenital abnormalities.

    N Tatsumi, N Hojo, O Yamada, M Ogawa, Y Katsura, S Kawata, E Morii, H Sakamoto, R Inaba, A Tsuda, I Fukuda, N Moriguchi, H Hasuwa, M Okabe, F Fujiki, S Nishida, H Nakajima, A Tsuboi, Y Oka, N Hosen, H Sugiyama and Y Oji

    Oncogene, 35 (8), 1003-1014, 2016.
29. Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization.

    Yuko Muro, Hidetoshi Hasuwa, Ayako Isotani, Haruhiko Miyata, Kazuo Yamagata, Masahito Ikawa, Ryuzo Yanagimachi and Masaru Okabe

    Biol Reprod, 94 (4), 80, 2016.
30. Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice.

    Masanaga Muto, Yoshitaka Fujihara, Tomohiro Tobita, Daiji Kiyozumi and Masahito Ikawa

    Biol Reprod, 94 (1), 6, 2016.

Link

Department of Experimental Genome Research

Animal Resource Center for Infectious Diseases

Research Institute for Microbial Diseases, Osaka University