Department of Experimental Genome Research

Staff:

Professor

Masahito Ikawa, Ph.D.

Specially Appointed Associate Professor(SUP)

Ayako Isotani, Ph.D.

Assistant Professor

Yuhkoh Satouh, Ph.D.

Yoshitaka Fujihara, Ph.D.

Haruhiko Miyata, Ph.D.

Masashi Mori, Ph.D.

Specially Appointed Assistant Professor(SUP)

Daiji Kiyozumi, Ph.D.

Specially Appointed Researcher

Taichi Noda, Ph.D.

Qian Zhang, Ph.D.

Educational Support Staff

Kazuhiro Kaseda

Technical Staff

Ryuta Inoue

Specially Appointed Technical Staff

Saori Haga

Mutsumi Yamane

Graduate Student

Kaori Nozawa, D.V.M.

Masanaga Muto

Asami Oji

Samantha Young

Daisuke Mashiko

Masaki Ogawa

Tomohiro Tobita

Hirotaka Kato

Takafumi Matsumura

Ferheen Abbasi

Subjects:

1. Analysis of molecular mechanisms involved in mammalian gametogenesis, fertilization, and implantation.
2. Development of new technologies to produce genetically manipulated animals. (CRISPR/Cas system, lentiviral vectors, etc.).
3. Analysis of interspecies cell-cell communication in xenogenic chimeric animals.

Papers published in 2015

1. Genetic dissection of pheromone processing reveals main olfactory system-mediated social behaviors in mice.

   Tomohiko Matsuo, Tatsuya Hattori, Akari Asaba, Naokazu Inoue, Nobuhiro Kanomata, Takefumi Kikusui, Reiko Kobayakawa and Ko Kobayakawa

   Proc Natl Acad Sci U S A, 112 (3), E311-320, 2015.
2. Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9.

   Arata Honda, Michiko Hirose, Tadashi Sankai, Lubna Yasmin, Kazuaki Yuzawa, Kimiko Honsho, Haruna Izu, Atsushi Iguchi, Masahito Ikawa and Atsuo Ogura

   Exp Anim, 64 (1), 31-37, 2015.
3. Pluripotent Stem Cells Derived From Mouse Primordial Germ Cells by Small Molecule Compounds.

   Tohru Kimura, Yoshiaki Kaga, Yoichi Sekita, Keita Fujikawa, Tsunetoshi Nakatani, Mika Odamoto, Soichiro Funaki, Masahito Ikawa, Kuniya Abe and Toru Nakano

   Stem Cells, 33 (1), 45-55, 2015.
4. Induction of DNA methylation by artificial piRNA production in male germ cells.

   Daisuke Itou, Yusuke Shiromoto, Shin-ya Yukiho, Chika Ishii, Toru Nishimura, Narumi Ogonuki, Atsuo Ogura, Hidetoshi Hasuwa, Yoshitaka Fujihara, Satomi Kuramochi-Miyagawa and Toru Nakano

   Curr Biol, 25 (7), 901-906, 2015.
5. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).

   Masato Ohtsuka, Hiromi Miura, Keiji Mochida, Michiko Hirose, Ayumi Hasegawa, Atsuo Ogura, Ryuta Mizutani, Minoru Kimura, Ayako Isotani, Masahito Ikawa, Masahiro Sato and Channabasavaiah B Gurumurthy

   BMC Genomics, 16, 274, 2015.
6. A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis.

   Hidenobu Okuda, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Akira Tsujimura, Norio Nonomura, Haruhiko Miyata, Masaru Okabe, Masahito Ikawa, Yoshitaka Kawakami, Naoki Goshima, Morimasa Wada and Hiromitsu Tanaka

   PLoS One, 10 (4), e0124293, 2015.
7. Quantitative assessment of telomerase components in cancer cell lines.

   Semih Can Akıncılar, Kee Chung Low, Chia Yi Liu, Ting Dong Yan, Asami Oji, Masahito Ikawa, Shang Li and Vinay Tergaonkar

   FEBS Lett, 589 (9), 974-984, 2015.
8. DDX60 Is Involved in RIG-I-Dependent and Independent Antiviral Responses, and Its Function Is Attenuated by Virus-Induced EGFR Activation.

   Hiroyuki Oshiumi, Moeko Miyashita, Masaaki Okamoto, Yuka Morioka, Masaru Okabe, Misako Matsumoto and Tsukasa Seya

   Cell Rep, 11 (8), 1193-1207, 2015.
9. APJ Regulates Parallel Alignment of Arteries and Veins in the Skin.

   Hiroyasu Kidoya, Hisamichi Naito, Fumitaka Muramatsu, Daishi Yamakawa, Weizhen Jia, Masahito Ikawa, Takashi Sonobe, Hirotsugu Tsuchimochi, Mikiyasu Shirai, Ralf H Adams, Akiyoshi Fukamizu and Nobuyuki Takakura

   Dev Cell, 33 (3), 247-259, 2015.
10. Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes.

    Ryuichi Ono, Masayuki Ishii, Yoshitaka Fujihara, Moe Kitazawa, Takako Usami, Tomoko Kaneko-Ishino, Jun Kanno, Masahito Ikawa and Fumitoshi Ishino

    Sci Rep, 5, 12281, 2015.
11. Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models.

    Samantha A M Young, R John Aitken and Masahito Ikawa

    Asian J Androl, 17 (4), 623-627, 2015.
12. Calreticulin is required for development of the cumulus oocyte complex and female fertility.

    Keizo Tokuhiro, Yuhkoh Satouh, Kaori Nozawa, Ayako Isotani, Yoshitaka Fujihara, Yumiko Hirashima, Hiroyuki Matsumura, Kazuhiro Takumi, Takashi Miyano, Masaru Okabe, Adam M Benham and Masahito Ikawa

    Sci Rep, 5, 14254, 2015.
13. Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche.

    Masahiko Yamaguchi, Yoko Watanabe, Takuji Ohtani, Akiyoshi Uezumi, Norihisa Mikami, Miki Nakamura, Takahiko Sato, Masahito Ikawa, Mikio Hoshino, Kunihiro Tsuchida, Yuko Miyagoe-Suzuki, Kazutake Tsujikawa, Shin'ichi Takeda, Hiroshi Yamamoto and So-ichiro Fukada

    Cell Rep, 13 (2), 302-314, 2015.
14. CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis.

    Samantha A M Young, Haruhiko Miyata, Yuhkoh Satouh, Hirotaka Kato, Kaori Nozawa, Ayako Isotani, R John Aitken, Mark A Baker and Masahito Ikawa

    Int J Mol Sci, 16 (10), 24732-24750, 2015.
15. Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive.

    Haruhiko Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka Fujihara, Ayako Isotani, Kazuo Inaba and Masahito Ikawa

    Science, 350 (6259), 442-445, 2015.
16. Sperm Postacrosomal WW Domain-Binding Protein Is Not Required for Mouse Egg Activation.

    Yuhkoh Satouh, Kaori Nozawa and Masahito Ikawa

    Biol Reprod, 93 (4), 94, 2015.
17. A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility.

    Sayuri Gyobu, Haruhiko Miyata, Masahito Ikawa, Daiju Yamazaki, Hiroshi Takeshima, Jun Suzuki and Shigekazu Nagata

    Mol Cell Biol, 36 (4), 645-659, 2015.
18. Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.

    Paulina A Latos, Arnold R Sienerth, Alexander Murray, Claire E Senner, Masanaga Muto, Masahito Ikawa, David Oxley, Sarah Burge, Brian J Cox and Myriam Hemberger

    Genes Dev, 29 (23), 2435-2448, 2015.

Link

Department of Experimental Genome Research

Animal Resource Center for Infectious Diseases

Research Institute for Microbial Diseases, Osaka University