Department of Experimental Genome Research

Staff:

Professor

Masahito Ikawa, Ph.D.

Specially Appointed Associate Professor (SUP)

Ayako Isotani, Ph.D.

Assistant Professor

Yuhkoh Satouh, Ph.D.

Yoshitaka Fujihara, Ph.D.

Haruhiko Miyata, Ph.D.

Masashi Mori, Ph.D.

Specially Appointed Assistant Professor (SUP)

Daiji Kiyozumi, Ph.D.

Specially Appointed Researcher

Taichi Noda, Ph.D.

Qian Zhang, Ph.D.

Educational Support Staff

Kazuhiro Kaseda

Technical Staff

Ryuta Inoue

Specially Appointed Technical Staff (SUP)

Saori Haga

Mutsumi Yamane

Graduate Student

Kaori Nozawa, D.V.M.

Masanaga Muto

Asami Oji

Samantha Young

Daisuke Mashiko

Masaki Ogawa

Hirotaka Kato

Takafumi Matsumura

Subjects:

1. Analysis of molecular mechanisms involved in mammalian gamategenesis, fertilization, and implantation.
2. Development of new technologies to produce genetically manipulated animals. (CRISPR/Cas system, lentiviral vectors, etc.)
3. Analysis of interspecies cell-cell communication in xenogenic chimeric animals.

Papers published in 2014

1. Rhof promotes murine marginal zone B cell development.

   Mayuko Kishimoto, Takenori Matsuda, Shougo Yanase, Akira Katsumi, Nobuaki Suzuki, Makoto Ikejiri, Akira Takagi, Masahito Ikawa, Tetsuhito Kojima, Shinji Kunishima, Hitoshi Kiyoi, Tomoki Naoe, Tadashi Matsushita and Mitsuo Maruyama

   Nagoya J Med Sci, 76 (3-4), 293-305, 2014.
2. CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection.

   Yoshitaka Fujihara and Masahito Ikawa

   Methods Enzymol, 546, 319-336, 2014.
3. Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury.

   Mika Takarada-Iemata, Dai Kezuka, Toshiaki Takeichi, Masahito Ikawa, Tsuyoshi Hattori, Yasuko Kitao and Osamu Hori

   J Neurochem, 130 (3), 374-387, 2014.
4. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.

   Seiya Mizuno, Tra Thi Huong Dinh, Kanako Kato, Saori Mizuno-Iijima, Yoko Tanimoto, Yoko Daitoku, Yoshikazu Hoshino, Masahito Ikawa, Satoru Takahashi, Fumihiro Sugiyama and Ken-Ichi Yagami

   Mamm Genome, 25 (7-8), 327-334, 2014.
5. NUCKS is a positive transcriptional regulator of insulin signaling.

   Beiying Qiu, Xiaohe Shi, Ee Tsin Wong, Joy Lim, Marco Bezzi, Diana Low, Qiling Zhou, Semih Can Akıncılar, Manikandan Lakshmanan, Hannah L F Swa, Jill Mae Lan Tham, Jayantha Gunaratne, Kenneth K Y Cheng, Wanjin Hong, Karen S L Lam, Masahito Ikawa, Ernesto Guccione, Aimin Xu, Weiping Han and Vinay Tergaonkar

   Cell Rep, 7 (6), 1876-1886, 2014.
6. Effect of isolated AMP deaminase deficiency on skeletal muscle function.

   J Chenga, H Morisaki, N Sugimoto, A Dohi, T Shintani, E Kimura, K Toyama, M Ikawa, M Okabe, I Higuchi, S Matsuo, Y Kawai, I Hisatome, T Sugama, E. W Holmes and T Morisaki

   Molecular Genetics and Metabolism Reports, 1, 51-59, 2014.
7. Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes.

   Daisuke Mashiko, Samantha A M Young, Masanaga Muto, Hirotaka Kato, Kaori Nozawa, Masaki Ogawa, Taichi Noda, Yeon-Joo Kim, Yuhkoh Satouh, Yoshitaka Fujihara and Masahito Ikawa

   Dev Growth Differ, 56 (1), 122-129, 2014.
8. Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.

   Kotaro Azuma, Tohru Tsukui, Kazuhiro Ikeda, Sachiko Shiba, Kimie Nakagawa, Toshio Okano, Tomohiko Urano, Kuniko Horie-Inoue, Yasuyoshi Ouchi, Masahito Ikawa and Satoshi Inoue

   PLoS One, 9 (2), e88643, 2014.
9. GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice.

   Yoshitaka Fujihara, Masaru Okabe and Masahito Ikawa

   Biol Reprod, 90 (3), 60, 2014.

Link

Department of Experimental Genome Research

Animal Resource Center for Infectious Diseases

Research Institute for Microbial Diseases, Osaka University