Center for Genetic Analysis of Biological Responses

Staff:

<Production laboratory for Genetically-manipulated Animals>

Professor(SUP)

Masahito Ikawa,Ph.D.

Specially Appointed Associate Professor

Kazuo Yamagata,Ph.D.

Specially Appointed Assistant Professor

Jun Ueda,Ph.D.

Specially Appointed Researcher

Masaru Okabe,Ph.D.

Mayuko Hori

<Resource laboratory for Genetically-manipulated Animals>

Specially Appointed Associate Professor(SUP)

Ayako Isotani,Ph.D.

Assistant Professor(SUP)

Haruhiko Miyata,Ph.D.

Specially Appointed Researcher

Kim Yeon Joo,Ph.D.

<Laboratory for Analysis of Genetically-manipulated Animals>

Professor(SUP)

Shizuo Akira,M.D.,Ph.D.

Hisashi Arase,M.D.,Ph.D.

Taroh Kinoshita,Ph.D.

Hitoshi Kikutani,M.D.,Ph.D.

Hiroshi Nojima,Ph.D.

Nobuyuki Takakura,M.D.,Ph.D.

Masato Okada,Ph.D.

Assistant Professor(SUP)

Yoshitaka Fujihara,Ph.D.

<Laboratory for Promotion of Collaborative Research>

Assistant Professor(SUP)

Naohisa Goto,Ph.D.

Yuhkoh Satouh,Ph.D.

Subjects:

1. Generation of the gene-manipulated animals for the study of reproduction, immunolgy, infecion and allergy.
2. Quantification of mouse preimplantation development using live-cell imaging technique.
3. Generation and application of epigenetic reporter mouse.

Paper published in 2014

1. Improved and robust detection of cell nuclei from four dimensional fluorescence images.

   Md Khayrul Bashar, Kazuo Yamagata and Tetsuya J Kobayashi

   PLoS One, 9 (7), e101891, 2014.
2. Heterochromatin Dynamics during the Differentiation Process Revealed by the DNA Methylation Reporter Mouse, MethylRO.

   Jun Ueda, Kazumitsu Maehara, Daisuke Mashiko, Takako Ichinose, Tatsuma Yao, Mayuko Hori, Yuko Sato, Hiroshi Kimura, Yasuyuki Ohkawa and Kazuo Yamagata

   Stem Cell Reports, 2 (6), 910-924, 2014.
   --
3. Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9.

   Arata Honda, Michiko Hirose, Tadashi Sankai, Lubna Yasmin, Kazuaki Yuzawa, Kimiko Honsho, Haruna Izu, Atsushi Iguchi, Masahito Ikawa and Atsuo Ogura

   Exp Anim, 64 (1), 31-37, 2015.
4. CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection.

   Yoshitaka Fujihara and Masahito Ikawa

   Methods Enzymol, 546, 319-336, 2014.
5. Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury.

   Mika Takarada-Iemata, Dai Kezuka, Toshiaki Takeichi, Masahito Ikawa, Tsuyoshi Hattori, Yasuko Kitao and Osamu Hori

   J Neurochem, 130 (3), 374-387, 2014.
6. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.

   Seiya Mizuno, Tra Thi Huong Dinh, Kanako Kato, Saori Mizuno-Iijima, Yoko Tanimoto, Yoko Daitoku, Yoshikazu Hoshino, Masahito Ikawa, Satoru Takahashi, Fumihiro Sugiyama and Ken-Ichi Yagami

   Mamm Genome, 25 (7-8), 327-334, 2014.
7. NUCKS is a positive transcriptional regulator of insulin signaling.

   Beiying Qiu, Xiaohe Shi, Ee Tsin Wong, Joy Lim, Marco Bezzi, Diana Low, Qiling Zhou, Semih Can Akıncılar, Manikandan Lakshmanan, Hannah L F Swa, Jill Mae Lan Tham, Jayantha Gunaratne, Kenneth K Y Cheng, Wanjin Hong, Karen S L Lam, Masahito Ikawa, Ernesto Guccione, Aimin Xu, Weiping Han and Vinay Tergaonkar

   Cell Rep, 7 (6), 1876-1886, 2014.
8. Effect of isolated AMP deaminase deficiency on skeletal muscle function.

   J Chenga, H Morisaki, N Sugimoto, A Dohi, T Shintani, E Kimura, K Toyama, M Ikawa, M Okabe, I Higuchi, S Matsuo, Y Kawai, I Hisatome, T Sugama, E. W Holmes and T Morisaki

   Molecular Genetics and Metabolism Reports, 1, 51-59, 2014.
9. Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes.

   Daisuke Mashiko, Samantha A M Young, Masanaga Muto, Hirotaka Kato, Kaori Nozawa, Masaki Ogawa, Taichi Noda, Yeon-Joo Kim, Yuhkoh Satouh, Yoshitaka Fujihara and Masahito Ikawa

   Dev Growth Differ, 56 (1), 122-129, 2014.
10. Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.

    Kotaro Azuma, Tohru Tsukui, Kazuhiro Ikeda, Sachiko Shiba, Kimie Nakagawa, Toshio Okano, Tomohiko Urano, Kuniko Horie-Inoue, Yasuyoshi Ouchi, Masahito Ikawa and Satoshi Inoue

    PLoS One, 9 (2), e88643, 2014.
11. GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice.

    Yoshitaka Fujihara, Masaru Okabe and Masahito Ikawa

    Biol Reprod, 90 (3), 60, 2014.
    --
12. Mechanism of fertilization: a modern view.

    Masaru Okabe

    Exp Anim, 63 (4), 357-365, 2014.
13. INAM plays a critical role in IFN-γ production by NK cells interacting with polyinosinic-polycytidylic acid-stimulated accessory cells.

    Jun Kasamatsu, Masahiro Azuma, Hiroyuki Oshiumi, Yuka Morioka, Masaru Okabe, Takashi Ebihara, Misako Matsumoto and Tsukasa Seya

    J Immunol, 193 (10), 5199-5207, 2014.
14. Induction of primordial germ cell-like cells from mouse embryonic stem cells by ERK signal inhibition.

    Tohru Kimura, Yoshiaki Kaga, Hiroshi Ohta, Mika Odamoto, Yoichi Sekita, Kunpeng Li, Noriko Yamano, Keita Fujikawa, Ayako Isotani, Norihiko Sasaki, Masashi Toyoda, Katsuhiko Hayashi, Masaru Okabe, Takashi Shinohara, Mitinori Saitou and Toru Nakano

    Stem Cells, 32 (10), 2668-2678, 2014.
15. Lessons Learned in Andrology: seeing is believing.

    M Okabe

    Andrology, 2 (1), 3-4, 2014.

Link

Center for Genetic Analysis of Biological Responses

Research Institute for Microbial Diseases, Osaka University