Department of Experimental Genome Research

Staff:

Professor

Masahito Ikawa, Ph.D.

Specially Appointed Associate Professor (SUP)

Ayako Isotani, Ph.D.

Assistant Professor

Yuhkoh Satouh, Ph.D.

Yoshitaka Fujihara, Ph.D.

Specially Appointed Assistant Professor (SUP)

Haruhiko Miyata, Ph.D.

Educational Support Staff

Kazuhiro Kaseda

Technical Staff

Ryuta Inoue

Specially Appointed Technical Staff (SUP)

Saori Haga

Mutsumi Yamane

Kazuta Mizuta

Graduate Student

Kaori Nozawa, D.V.M.

Masanaga Muto

Daisuke Mashiko

Masaki Ogawa

Undergraduate Student

Hirotaka Kato

Subjects:

1. Analysis of molecular mechanisms involved in mammalian gamategenesis, fertilization, and implantation.
2. Development of new technologies to produce genetically manipulated animals. (CRISPR/Cas system, lentiviral vectors, etc.)
3. Analysis of interspecies cell-cell communication in xenogenic chimeric animals.

Papers published in 2013

1. Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.

   Daisuke Mashiko, Yoshitaka Fujihara, Yuhkoh Satouh, Haruhiko Miyata, Ayako Isotani and Masahito Ikawa

   Sci Rep, 3, 3355, 2013.
2. Establishment of mouse model of MYH9 disorders: heterozygous R702C mutation provokes macrothrombocytopenia with leukocyte inclusion bodies, renal glomerulosclerosis and hearing disability.

   Nobuaki Suzuki, Shinji Kunishima, Makoto Ikejiri, Shoichi Maruyama, Michihiko Sone, Akira Takagi, Masahito Ikawa, Masaru Okabe, Tetsuhito Kojima, Hidehiko Saito, Tomoki Naoe and Tadashi Matsushita

   PLoS One, 8 (8), e71187, 2013.
3. Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma.

   Junko Tashiro-Yamaji, Shogo Maeda, Masahito Ikawa, Masaru Okabe, Takahiro Kubota and Ryotaro Yoshida

   Transplantation, 96 (3), 251-257, 2013.
4. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion.

   Naokazu Inoue, Daizo Hamada, Hironari Kamikubo, Kunio Hirata, Mikio Kataoka, Masaki Yamamoto, Masahito Ikawa, Masaru Okabe and Yoshihisa Hagihara

   Development, 140 (15), 3221-3229, 2013.
5. Targeted disruption of fad24, a regulator of adipogenesis, causes pre-implantation embryonic lethality due to the growth defect at the blastocyst stage.

   Natsuki Ochiai, Makoto Nishizuka, Tomomi Miyamoto, Ichiro Miyoshi, Masahito Ikawa, Shigehiro Osada and Masayoshi Imagawa

   Biochem Biophys Res Commun, 438 (2), 301-305, 2013.
6. miR-200b and miR-429 function in mouse ovulation and are essential for female fertility.

   Hidetoshi Hasuwa, Jun Ueda, Masahito Ikawa and Masaru Okabe

   Science, 341 (6141), 71-73, 2013.
7. Recruitment of cyclin G2 to promyelocytic leukemia nuclear bodies promotes dephosphorylation of γH2AX following treatment with ionizing radiation.

   Yoko Naito, Norikazu Yabuta, Jun Sato, Shouichi Ohno, Muneki Sakata, Takashi Kasama, Masahito Ikawa and Hiroshi Nojima

   Cell Cycle, 12 (11), 1773-1784, 2013.
8. Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa.

   Yoshitaka Fujihara, Keizo Tokuhiro, Yuko Muro, Gen Kondoh, Yoshihiko Araki, Masahito Ikawa and Masaru Okabe

   Proc Natl Acad Sci U S A, 110 (20), 8111-8116, 2013.
9. A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration.

   Satoshi Nojima, Toshihiko Toyofuku, Hiroyuki Kamao, Chie Ishigami, Jun Kaneko, Tatsusada Okuno, Hyota Takamatsu, Daisuke Ito, Sujin Kang, Tetsuya Kimura, Yuji Yoshida, Keiko Morimoto, Yohei Maeda, Atsushi Ogata, Masahito Ikawa, Eiichi Morii, Katsuyuki Aozasa, Junichi Takagi, Masayo Takahashi and Atsushi Kumanogoh

   Nat Commun, 4, 1406, 2013.
10. Production of mouse pups from germline transmission-failed knockout chimeras.

    Yoshitaka Fujihara, Kazuhiro Kaseda, Naokazu Inoue, Masahito Ikawa and Masaru Okabe

    Transgenic Res, 22 (1), 195-200, 2013.
11. Neutrophil infiltration during inflammation is regulated by PILRα via modulation of integrin activation.

    Jing Wang, Ikuo Shiratori, Junji Uehori, Masahito Ikawa and Hisashi Arase

    Nat Immunol, 14 (1), 34-40, 2013.
12. N-terminal truncation of Lats1 causes abnormal cell growth control and chromosomal instability.

    Norikazu Yabuta, Satomi Mukai, Ayumi Okamoto, Daisuke Okuzaki, Hirokazu Suzuki, Kosuke Torigata, Kaori Yoshida, Nobuhiro Okada, Daisaku Miura, Akihiko Ito, Masahito Ikawa, Masaru Okabe and Hiroshi Nojima

    J Cell Sci, 126 (Pt 2), 508-520, 2013.

Link

http://www.biken.osaka-u.ac.jp/