Department of Immunoregulation

Subjects:

1. Biochemistry and molecular biology of biosynthesis pathway of GPI-anchored proteins.
2. Molecular pathogenesis of a GPI-anchor deficiencies, paroxysmal nocturnal hemoglobinuria and inherited GPI deficiency.
3. Roles of GPI in protozoan parasites.

Published Papers

1. Isolation of new CHO cell mutants defective in CMP-sialic acid biosynthesis and transport.

   Dong-Jun Shin, Ji Young Kang, Youn Uck Kim, Joong Sik Yoon, Hyon E Choy, Yusuke Maeda, Taroh Kinoshita and Yeongjin Hong

   Mol Cells, 22 (3), 343-352, 2006.
2. CHO Glycosylation Mutants: GPI Anchor.

   Yusuke Maeda, Hisashi Ashida and Taroh Kinoshita

   Methods Enzymol, 416, 182-205, 2006.
3. Molecular basis of clonal expansion of hematopoiesis in two patients with paroxysmal nocturnal hemoglobinuria (PNH).

   Norimitsu Inoue, Tomohisa Izui-Sarumaru, Yoshiko Murakami, Yuichi Endo, Jun-Ichi Nishimura, Ken Kurokawa, Maki Kuwayama, Hiroaki Shime, Takashi Machii, Yuzuru Kanakura, Gabrielle Meyers, Carl Wittwer, Zhong Chen, William Babcock, Debra Frei-Lahr, Charles J Parker and Taroh Kinoshita

   Blood, 108 (13), 4232-4236, 2006.
4. PimE Is a Polyprenol-phosphate-mannose-dependent Mannosyltransferase That Transfers the Fifth Mannose of Phosphatidylinositol Mannoside in Mycobacteria.

   Yasu S Morita, Chubert B C Sena, Ross F Waller, Ken Kurokawa, M Fleur Sernee, Fumiki Nakatani, Ruth E Haites, Helen Billman-Jacobe, Malcolm J McConville, Yusuke Maeda and Taroh Kinoshita

   J Biol Chem, 281 (35), 25143-25155, 2006.
5. Hypomorphic promoter mutation in PIGM causes inherited glycosylphosphatidylinositol deficiency.

   Antonio M Almeida, Yoshiko Murakami, D Mark Layton, Peter Hillmen, Gabrielle S Sellick, Yusuke Maeda, Stephen Richards, Scott Patterson, Ioannis Kotsianidis, Luigina Mollica, Dorothy H Crawford, Alastair Baker, Michael Ferguson, Irene Roberts, Richard Houlston, Taroh Kinoshita and Anastasios Karadimitris

   Nat Med, 12 (7), 846-851, 2006.
6. Removal or maintenance of inositol-linked acyl chain in GPI is criticalin trypanosome life cycle.

   Yeonchul Hong, Kisaburo Nagamune, Yasu S Morita, Fumiki Nakatani, Hisashi Ashida, Yusuke Maeda and Taroh Kinoshita

   J Biol Chem, 281 (17), 11595-11602, 2006.
7. PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins.

   Yuko Tashima, Ryo Taguchi, Chie Murata, Hisashi Ashida, Taroh Kinoshita and Yusuke Maeda

   Mol Biol Cell, 17 (3), 1410-1420, 2006.
8. TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei.

   Yeonchul Hong, Kisaburo Nagamune, Kazuhito Ohishi, Yasu S Morita, Hisashi Ashida, Yusuke Maeda and Taroh Kinoshita

   FEBS Lett, 580 (2), 603-606, 2006.
9. DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3.

   Hisashi Ashida, Yusuke Maeda and Taroh Kinoshita

   J Biol Chem, 281 (2), 896-904, 2006.
10. A cohort study of the nature of paroxysmal nocturnal hemoglobinuria clones and PIG-A mutations in patients with aplastic anemia.

    Wanchai Wanachiwanawin, Uamporn Siripanyaphinyo, Nattawan Piyawattanasakul and Taroh Kinoshita

    Eur J Haematol, 76 (6), 502-509, 2006.

Link

http://www.biken.osaka-u.ac.jp/