Department of Experimental Genome Research

Subjects:

1. Analysis of Molecular mechanisms involved in reproduction using genetically modified animals.
2. Development of new technologies to produce genetically manipulated animals by using Lentiviral vectors and RNA interference.
3. Analysis of sex differentiation of germ cells and preimplantation embryos.
4. Investigation of the molecular biological mechanisms involved in cardiovascular diseases.

Published Papers

1. Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.

   Shin Kobayashi, Ayako Isotani, Nathan Mise, Masamichi Yamamoto, Yoshitaka Fujihara, Kazuhiro Kaseda, Tomoko Nakanishi, Masahito Ikawa, Hiroshi Hamada, Kuniya Abe and Masaru Okabe

   Curr Biol, 16 (2), 166-172, 2006.
2. Role of the protomap and target-derived signals in the development of intrahemispheric connections.

   Wanzhu Bai, Mami Ishida, Masaru Okabe and Yasuyoshi Arimatsu

   Cereb Cortex, 16 (1), 124-135, 2006.
3. Activation of Akt signaling is sufficient to maintain pluripotency in mouse and primate embryonic stem cells.

   S Watanabe, H Umehara, K Murayama, M Okabe, T Kimura and T Nakano

   Oncogene, 25 (19), 2697-2707, 2006.
4. RECS1 deficiency in mice induces susceptibility to cystic medial degeneration.

   Hanjun Zhao, Akihiko Ito, Shinya H Kimura, Norikazu Yabuta, Naohiko Sakai, Masahito Ikawa, Masaru Okabe, Yuji Matsuzawa, Shizuya Yamashita and Hiroshi Nojima

   Genes Genet Syst, 81 (1), 41-50, 2006.
5. Adenovirus serotype 35 vector-mediated transduction into human CD46-transgenic mice.

   F Sakurai, K Kawabata, N Koizumi, N Inoue, M Okabe, T Yamaguchi, T Hayakawa and H Mizuguchi

   Gene Ther, 13 (14), 1118-1126, 2006.
6. Plexin-A1 and its interaction with DAP12 in immune responses and bone homeostasis.

   Noriko Takegahara, Hyota Takamatsu, Toshihiko Toyofuku, Tohru Tsujimura, Tatsusada Okuno, Kazunori Yukawa, Masayuki Mizui, Midori Yamamoto, Durbaka V R Prasad, Kazuhiro Suzuki, Masaru Ishii, Kenta Terai, Masayuki Moriya, Yuji Nakatsuji, Saburo Sakoda, Shintaro Sato, Shizuo Akira, Kiyoshi Takeda, Masanori Inui, Toshiyuki Takai, Masahito Ikawa, Masaru Okabe, Atsushi Kumanogoh and Hitoshi Kikutani

   Nat Cell Biol, 8 (6), 615-622, 2006.
7. Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice.

   Ryo Yamaguchi, Kazuo Yamagata, Masahito Ikawa, Stuart B Moss and Masaru Okabe

   Biol Reprod, 75 (5), 760-766, 2006.
8. Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells.

   Mito Kanatsu-Shinohara, Masahito Ikawa, Masanori Takehashi, Narumi Ogonuki, Hiromi Miki, Kimiko Inoue, Yasuhiro Kazuki, Jiyoung Lee, Shinya Toyokuni, Mitsuo Oshimura, Atsuo Ogura and Takashi Shinohara

   Proc Natl Acad Sci U S A, 103 (21), 8018-8023, 2006.
9. Choroidal neovascularization in transgenic mice expressing prokineticin 1: an animal model for age-related macular degeneration.

   Nobushige Tanaka, Masahito Ikawa, Nathan L Mata and Inder M Verma

   Mol Ther, 13 (3), 609-616, 2006.
10. Smooth muscle alpha-actin deficiency in myofibroblasts leads to enhanced renal tissue fibrosis.

    Masanobu Takeji, Toshiki Moriyama, Susumu Oseto, Noritaka Kawada, Masatsugu Hori, Enyu Imai and Takeshi Miwa

    J Biol Chem, 281 (52), 40193-40200, 2006.
11. Smooth muscle-specific dystrophin expression improves aberrant vasoregulation in mdx mice.

    Kaori Ito, Shigemi Kimura, Shiro Ozasa, Makoto Matsukura, Makoto Ikezawa, Kowashi Yoshioka, Hiroe Ueno, Misao Suzuki, Kimi Araki, Ken-ichi Yamamura, Takeshi Miwa, George Dickson, Gail D Thomas and Teruhisa Miike

    Hum Mol Genet, 15 (14), 2266-2275, 2006.
12. Mitochondria-related male infertility.

    Kazuto Nakada, Akitsugu Sato, Kayo Yoshida, Takashi Morita, Hiromitsu Tanaka, Shin-Ichi Inoue, Hiromichi Yonekawa and Jun-Ichi Hayashi

    Proc Natl Acad Sci U S A, 103 (41), 15148-15153, 2006.
13. A novel mammalian bubblegum-related acyl-CoA synthetase restricted to testes and possibly involved in spermatogenesis.

    Peter Fraisl, Hiromitsu Tanaka, Sonja Forss-Petter, Hans Lassmann, Yoshitake Nishimune and Johannes Berger

    Arch Biochem Biophys, 451 (1), 23-33, 2006.
14. Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein.

    Hiromitsu Tanaka, Yasuhiro Matsuoka, Masayoshi Onishi, Kouichi Kitamura, Yasushi Miyagawa, Hiromi Nishimura, Akira Tsujimura, Akihiko Okuyama and Yoshitake Nishimune

    Int J Androl, 29 (2), 353-359, 2006.
15. Expression of Mina53, a product of a Myc target gene in mouse testis.

    Makoto Tsuneoka, Yoshitake Nishimune, Keisuke Ohta, Kwesi Teye, Hiromitsu Tanaka, Mikiko Soejima, Hiroshi Iida, Tetsuo Inokuchi, Hiroshi Kimura and Yoshiro Koda

    Int J Androl, 29 (2), 323-330, 2006.
16. Infertility caused by polymorphisms or mutations in spermatogenesis-specific genes.

    Yoshitake Nishimune and Hiromitsu Tanaka

    J Androl, 27 (3), 326-334, 2006.

Link

http://www.biken.osaka-u.ac.jp/