Department of Science for Laboratory Animal Experimentation

Subjects:

1. Studies on the molecular mechanism of male germ-cell differentiation.
2. Studies on the property of the germinal stem cells.
3. Studies on the human male infertility.

Papers published in 2003

1. Differential expression of succinyl CoA transferase (SCOT) genes in somatic and germline cells of the mouse testis.

   H Tanaka, N Iguchi, Y Miyagawa, M Koga, J Kohroki and Y Nishimune

   Int J Androl, 26 (1), 52-56, 2003.
2. Mouse germ cell-less as an essential component for nuclear integrity.

   Tohru Kimura, Chizuru Ito, Shoko Watanabe, Tohru Takahashi, Masahito Ikawa, Kentaro Yomogida, Yukiko Fujita, Megumi Ikeuchi, Noriko Asada, Kiyomi Matsumiya, Akihiko Okuyama, Masaru Okabe, Kiyotaka Toshimori and Toru Nakano

   Mol Cell Biol, 23 (4), 1304-1315, 2003.
3. Single nucleotide polymorphisms in the protamine-1 and -2 genes of fertile and infertile human male populations.

   Hiromitsu Tanaka, Yasushi Miyagawa, Akira Tsujimura, Kiyomi Matsumiya, Akihiko Okuyama and Yoshitake Nishimune

   Mol Hum Reprod, 9 (2), 69-73, 2003.
4. Functional analysis of the p53 gene in apoptosis induced by heat stress or loss of stem cell factor signaling in mouse male germ cells.

   Hiroshi Ohta, Shinichi Aizawa and Yoshitake Nishimune

   Biol Reprod, 68 (6), 2249-2254, 2003.
5. Conditional loss of PTEN leads to testicular teratoma and enhances embryonic germ cell production.

   Tohru Kimura, Akira Suzuki, Yukiko Fujita, Kentaro Yomogida, Hilda Lomeli, Noriko Asada, Megumi Ikeuchi, Andras Nagy, Tak W Mak and Toru Nakano

   Development, 130 (8), 1691-1700, 2003.
6. A novel protein phosphatase 2C family member (PP2Czeta) is able to associate with ubiquitin conjugating enzyme 9.

   Mitsuhiro Kashiwaba, Koji Katsura, Motoko Ohnishi, Mutsuo Sasaki, Hiromitsu Tanaka, Yoshitake Nishimune, Takayasu Kobayashi and Shinri Tamura

   FEBS Lett, 538 (1-3), 197-202, 2003.
7. The role of the c-kit receptor in the regenerative differentiation of rat Leydig cells.

   Junji Tsuchida, Kayoko Dohmae, Yukihiko Kitamura and Yoshitake Nishimune

   Int J Androl, 26 (2), 121-125, 2003.
8. Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells.

   Hiromitsu Tanaka, Naoko Iguchi, Carlos Egydio de Carvalho, Yuko Tadokoro, Kentaro Yomogida and Yoshitake Nishimune

   Biol Reprod, 69 (2), 475-482, 2003.
9. Genomic structure and promoter activity of the testis haploid germ cell-specific intronless genes, Tact1 and Tact2.

   Mizue Hisano, Shuichi Yamada, Hiromitsu Tanaka, Yoshitake Nishimune and Masami Nozaki

   Mol Reprod Dev, 65 (2), 148-156, 2003.
10. Molecular cloning and characterization of the human orthologue of the oppo 1 gene encoding a sperm tail protein.

    Kouichi Kitamura, Yasushi Miyagawa, Naoko Iguchi, Hiromi Nishimura, Hiromitsu Tanaka and Yoshitake Nishimune

    Mol Hum Reprod, 9 (5), 237-243, 2003.
11. Involvement of death receptor Fas in germ cell degeneration in gonads of Kit-deficient Wv/Wv mutant mice.

    S Sakata, K Sakamaki, K Watanabe, N Nakamura, S Toyokuni, Y Nishimune, C Mori and S Yonehara

    Cell Death Differ, 10 (6), 676-686, 2003.
12. Transcriptional regulation of the cytosolic chaperonin theta subunit gene, Cctq, by Ets domain transcription factors Elk-1, Sap-1a, and Net in the absence of serum response factor.

    Yuji Yamazaki, Hiroshi Kubota, Masami Nozaki and Kazuhiro Nagata

    J Biol Chem, 278 (33), 30642-30651, 2003.
13. Expression of Tom34 splicing isoforms in mouse testis and knockout of Tom34 in mice.

    Kazutoyo Terada, Shota Ueno, Kentaro Yomogida, Tomoaki Imai, Hiroshi Kiyonari, Naoki Takeda, Masato Yano, Shinichi Abe, Shinichi Aizawa and Masataka Mori

    J Biochem (Tokyo), 133 (5), 625-631, 2003.
14. Characterization of histone H2A.X expression in testis and specific labeling of germ cells at the commitment stage of meiosis with histone H2A.X promoter-enhanced green fluorescent protein transgene.

    Yuko Tadokoro, Kentaro Yomogida, Yo Yagura, Shuichi Yamada, Masaru Okabe and Yoshitake Nishimune

    Biol Reprod, 69 (4), 1325-1329, 2003.
15. Dramatic expansion of germinal stem cells by ectopically expressed human glial cell line-derived neurotrophic factor in mouse Sertoli cells.

    Kentaro Yomogida, Yo Yagura, Yuko Tadokoro and Yoshitake Nishimune

    Biol Reprod, 69 (4), 1303-1307, 2003.
16. GATA-1 testis activation region is essential for Sertoli cell-specific expression of GATA-1 gene in transgenic mouse.

    Junko Wakabayashi, Kentaro Yomogida, Osamu Nakajima, Keigyou Yoh, Satoru Takahashi, James Douglas Engel, Kinuko Ohneda and Masayuki Yamamoto

    Genes Cells, 8 (7), 619-630, 2003.
17. Proliferation and differentiation of spermatogonial stem cells in the w/wv mutant mouse testis.

    Hiroshi Ohta, Akira Tohda and Yoshitake Nishimune

    Biol Reprod, 69 (6), 1815-1821, 2003.
18. Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells.

    Mizue Hisano, Hiroshi Ohta, Yoshitake Nishimune and Masami Nozaki

    Nucleic Acids Res, 31 (16), 4797-4804, 2003.
19. Haprin, a novel haploid germ cell-specific RING finger protein involved in the acrosome reaction.

    Kouichi Kitamura, Hiromitsu Tanaka and Yoshitake Nishimune

    J Biol Chem, 278 (45), 44417-44423, 2003.
20. In vitro and in vivo gene delivery by recombinant baculoviruses.

    Hideki Tani, Chang Kwang Limn, Chan Choo Yap, Masayoshi Onishi, Masami Nozaki, Yoshitake Nishimune, Nobuo Okahashi, Yoshinori Kitagawa, Rie Watanabe, Rika Mochizuki, Kohji Moriishi and Yoshiharu Matsuura

    J Virol, 77 (18), 9799-9808, 2003.
21. Isolation and characterization of a novel cDNA encoding a DNA-binding protein (Hils1) specifically expressed in testicular haploid germ cells.

    Naoko Iguchi, Hiromitsu Tanaka, Kentaro Yomogida and Yoshitake Nishimune

    Int J Androl, 26 (6), 354-365, 2003.

Link

http://www.biken.osaka-u.ac.jp/